In
vitro
Efficacy of Areca catechu
against Cypermethrin Resistant
Rhipicephalus microplus and its Phytochemical Analysis
Muhammad Usman Naseer1, Zia ud Din
Sindhu1*, Zafar Iqbal1 and Bilal Aslam2
1Department
of Parasitology, University of Agriculture, Faisalabad-38040,
Pakistan
2Institute
of Physiology and Pharmacology, University of Agriculture,
Faisalabad-38040, Pakistan
*Corresponding author:
ziasandhu@hotmail.com; sandhu@uaf.edu.pk
Abstract
This study investigated the use of the crude
extract alongwith, water and petroleum ether fractions of
Areca (A.)
catechu seeds against cypermethrin resistant
Rhipicephalus (R.)
microplus. A colony of cypermethrin-resistant
R. microplus was maintained on cattle
calves. Areca catechu seeds were purchased from the local market of
Faisalabad. The crude aqueous methanol extract (CAME) and two fractions were
prepared using standard phytochemical procedures. All these extracts/fractions
were used to check their efficacy against cypermethrin-resistant
R. microplus by using the syringe test (Modified Larval Immersion
Test). Mortality (%) results obtained in the syringe test were subjected to the
Probit (dose-response) analysis and phytochemical analysis was performed to
check the presence of active group of compounds. Crude extract and two fractions of
A. catechu were found to be effective
against cypermethrin resistant R.
microplus. The most effective fraction of
A. catechu was petroleum ether fraction with LC50 = 0.3,
LC90 = 0.8 and LC99 = 1.7. Results of Petroleum ether
fraction were significantly different (p <0.05) from other two tested fractions.
In the phytochemical analysis carbohydrates, alkaloids, flavonoids, tannins,
phenols, glycosides, volatile oils and saponins were found to be the group of
compounds. These results indicated that
A. catechu has acaricidal potential
against cypermethrin-resistant R.
microplus.
To Cite This Article:
Naseer MU, Sindhu ZuD, Iqbal Z, Aslam B 2022.
In vitro efficacy of
Areca catechu against cypermethrin
resistant Rhipicephalus microplus and
its phytochemical analysis. Pak Vet J, 42(3):
414-418.
http://dx.doi.org/10.29261/pakvetj/2020.053