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Epidemiological Investigation and Development of Loop Mediated Isothermal Amplification for the Diagnosis of Ovine Theileriosis
Muhammad Arfan Zaman1*, Azhar Rafique2, Uzma Mehreen1, Saba Mehnaz3, Farhan Ahmad Atif4, Asghar Abbas5, Kashif Hussain5, Muhammad Asif Raza5, Sidra Altaf6, Faisal Siddique7, Rahman Md. Masudur8 and Mosaab Omar9,10

1Department of Pathobiology, University of Veterinary and Animal Sciences, Lahore (Jhang Campus), Pakistan
2Department of Zoology, GC University Faisalabad, Pakistan; 3Department of Parasitology, University of Agriculture, Faisalabad, Pakistan; 4Department of Clinical Medicine and Surgery, University of Veterinary and Animal Sciences, Lahore, Pakistan; 5Department of Veterinary and Animal Sciences, Muhammad Nawaz Sharif University of Agriculture Multan, Pakistan; 6Department of Pharmacy, University of Agriculture, Faisalabad
Department of Microbiology, Cholistan University of Veterinary and Animal Sciences, Bahawalpur, Pakistan
Department of Pathology, Faculty of Veterinary, Animal and Biomedical Sciences, Sylhet Agricultural University, Sylhet-3100, Bangladesh
Department of Veterinary Medicine, College of Agriculture and Veterinary Medicine, Qassim University, 51452 Qassim, Saudi Arabia; 10Department of Parasitology, Faculty of Veterinary Medicine, South-Valley University, Qena 83523, Egypt
*Corresponding author:


Small ruminant industry is threatened in tropical and sub-tropical countries due to a Theileria (T.) lestoquardi, a haemoprotozoan. A Loop-Mediated Isothermal Amplification (LAMP) assay was developed for diagnosis of T. lestoquardi, a major cause of ovine theileriosis. The primers were designed based on the clone-5 sequence of T. lestoquardi. Four types of geo-climatic regions were selected for sampling. Towards this end, 56% sheep were infected with T. lestoquardi in the study area. 9.1% were found infected by using Giemsa staining technique, whereas, 13 and 17.1% by polymerase chain reaction (PCR) and loop mediated isothermal amplification (LAMP), respectively. PCR was considered a reference test to evaluate specificity and sensitivity of LAMP. The relative specificity and sensitivity of LAMP assay was 94.4 and 78%, respectively. Findings of LAMP assay revealed that the highest prevalence was detected in Bahawalpur (21.6% 19/88*100) followed in order by Jhang (17.04% 15/88*100), Sahiwal (15.09% 14/88*100) and Faisalabad (13.63% 12/88*100). LAMP assay found most suitable test for diagnosis of T. lestoquardi. This is anticipated that this study will provide a base-line data for caretakers and veterinary personnel to devise an improved scheme for the effective control of ovine theileriosis.

To Cite This Article: Zaman MA, Rafique A, Mehreen U, Mehnaz S, Atif FA, Abbas A, Hussain K, Raza MA, Altaf S, Siddique F, Masudur RMd, Omar M 2022. Epidemiological investigation and development of loop mediated isothermal amplification for the diagnosis of ovine theileriosis. Pak Vet J, 42(3): 370-375.


ISSN 0253-8318 (Print)
ISSN 2074-7764 (Online)