PAKISTAN VETERINARY JOURNAL

Pak Vet J, 2022, 42(3): 370-375

Epidemiological Investigation and Development of Loop Mediated Isothermal Amplification for the Diagnosis of Ovine Theileriosis

Muhammad Arfan Zaman¹*, Azhar Rafique², Uzma Mehreen¹, Saba Mehnaz³, Farhan Ahmad Atif⁴, Asghar Abbas⁵, Kashif Hussain⁵, Muhammad Asif Raza⁵, Sidra Altaf⁶, Faisal Siddique⁷, Rahman Md. Masudur⁸ and Mosaab Omar^9,10

¹Department of Pathobiology, University of Veterinary and Animal Sciences, Lahore (Jhang Campus), Pakistan
²Department of Zoology, GC University Faisalabad, Pakistan; ³Department of Parasitology, University of Agriculture, Faisalabad, Pakistan; ⁴Department of Clinical Medicine and Surgery, University of Veterinary and Animal Sciences, Lahore, Pakistan; ⁵Department of Veterinary and Animal Sciences, Muhammad Nawaz Sharif University of Agriculture Multan, Pakistan; ⁶Department of Pharmacy, University of Agriculture, Faisalabad⁷Department of Microbiology, Cholistan University of Veterinary and Animal Sciences, Bahawalpur, Pakistan⁸Department of Pathology, Faculty of Veterinary, Animal and Biomedical Sciences, Sylhet Agricultural University, Sylhet-3100, Bangladesh⁹Department of Veterinary Medicine, College of Agriculture and Veterinary Medicine, Qassim University, 51452 Qassim, Saudi Arabia; ¹⁰Department of Parasitology, Faculty of Veterinary Medicine, South-Valley University, Qena 83523, Egypt
*Corresponding author: arfan.zaman@uvas.edu.pk

Abstract

Small ruminant industry is threatened in tropical and sub-tropical countries due to a Theileria (T.) lestoquardi, a haemoprotozoan. A Loop-Mediated Isothermal Amplification (LAMP) assay was developed for diagnosis of T. lestoquardi, a major cause of ovine theileriosis. The primers were designed based on the clone-5 sequence of T. lestoquardi. Four types of geo-climatic regions were selected for sampling. Towards this end, 56% sheep were infected with T. lestoquardi in the study area. 9.1% were found infected by using Giemsa staining technique, whereas, 13 and 17.1% by polymerase chain reaction (PCR) and loop mediated isothermal amplification (LAMP), respectively. PCR was considered a reference test to evaluate specificity and sensitivity of LAMP. The relative specificity and sensitivity of LAMP assay was 94.4 and 78%, respectively. Findings of LAMP assay revealed that the highest prevalence was detected in Bahawalpur (21.6% 19/88*100) followed in order by Jhang (17.04% 15/88*100), Sahiwal (15.09% 14/88*100) and Faisalabad (13.63% 12/88*100). LAMP assay found most suitable test for diagnosis of T. lestoquardi. This is anticipated that this study will provide a base-line data for caretakers and veterinary personnel to devise an improved scheme for the effective control of ovine theileriosis.

To Cite This Article: Zaman MA, Rafique A, Mehreen U, Mehnaz S, Atif FA, Abbas A, Hussain K, Raza MA, Altaf S, Siddique F, Masudur RMd, Omar M 2022. Epidemiological investigation and development of loop mediated isothermal amplification for the diagnosis of ovine theileriosis. Pak Vet J, 42(3): 370-375. http://dx.doi.org/10.29261/pakvetj/2022.039

Abstract

ISSN 0253-8318 (Print)ISSN 2074-7764 (Online)

ISSN 0253-8318 (Print)
ISSN 2074-7764 (Online)