Resistance Modulation of Dairy Milk Borne
Streptococcus agalactiae and Klebsiella pneumoniae
through Metallic Oxide Nanoparticles
Aymen1, Naima1, Amjad Islam Aqib*2,
Kashif Akram1, Hamid Majeed1, Maheen Murtaza3,
Afshan Muneer3 and Abdullah F Alsayeqh*4
1Department
of Food Science, Cholistan University of Veterinary and Animal
Sciences, 63100, Bahawalpur
2Department
of Medicine, Cholistan University of Veterinary and Animal Sciences,
63100, Bahawalpur
3Department
of Zoology, Cholistan University of Veterinary and Animal Sciences,
63100, Bahawalpur 4Department of Veterinary
Medicine, College of Agriculture and Veterinary Medicine, Qassim
University, Buraidah 51452, Qassim, Kingdom of Saudi Arabia *Corresponding author:amjadislamaqib@cuvas.edu.pk;
a.alsayeqh@qu.edu.sa
Abstract
Dairy udder and milk
equally remain susceptible to bacterial contamination posing significant threat
to food safety and security. Adding to this is overlooking some of bacterial
pathogens e.g. K. pneumoniae and S. agalactiae for their
infection, drug resistance, and demand of alternative therapeutics. Keeping in
view the scenario, current study was planned to assess response of K.
pneumoniae and S. agalactiae
against wider range of antibiotics, and resistance modulation through metallic
oxide nanoparticles of iron, zinc, and magnesium. Nanoparticles were prepared
and characterized as per set protocols. In-vitro disc diffusion test for
antibiotics, well diffusion for nanoparticles’ antibacterial activity, and
finally finding minimum inhibitory concentration expressing antibacterial
activity was carried out. Parametric and nonparametric statistics was applied to
confer outcomes using SPSS version 22 of statistical software at 5% probability.
Study found 6 out of 16 antibiotics showing minor variation in means of ZOI
against K. pneumoniae and S. agalactiae
indicating limited scope of antibiotics to cover infection against these
bacteria. When these bacteria were tested against MgO, ZnO, and Fe2O3
nanoparticles, significant antibacterial activity was noted. Fe2O3
showed significant higher antibacterial activity as compared to others against
both of bacterial strains. Minimum time for maximum activity was found variable
for each of nanoparticles against K.
pneumoniae and S.
agalactiaewith a range of 8th-20th hour of
incubation as significant response compared to 4th hour of
incubation. Study thus concluded, narrow window of antibiotic susceptibility
against K. pneumoniae and S. agalactiaewhile
wider scope of metallic oxide nanoparticles as antibacterial alternatives.
To Cite This Article:
Aymen, Naima, Aqib AI,
Akram K, Majeed H, Murtaza M, Muneer A and Alsayeqh AF, 2022. Resistance modulation of dairy milk borne
Streptococcus agalactiae and Klebsiella pneumoniae through metallic
oxide nanoparticles. Pak Vet J, 42(3):
424-428.
http://dx.doi.org/10.29261/pakvetj/2022.052