The broiler performance can be influenced by optimizing the in-house environment and the negative effects of litter on the environment of the poultry can be minimized by the utilization of nanoparticles to reduce the ammonia production. For this purpose, this study was performed for 42 days, including 540-day-old broiler birds to ascertain the best levels of nanoparticles. The birds were randomly divided at day 15 into A-I groups, where A was as positive control, B as negative control, and C as starch treatment group. The groups D, E and F were divided into three subgroups. Birds were treated with three types of nanoparticles, i.e., alum, zinc oxide and copper oxide. Nanoparticles were sprayed on litter material to kill the litter microbes and reduce the ammonia emission. Ammonia was produced by maintaining wet litter conditions by sprinkling water at the rate of 250 ml per day for first week of treatment trial. Ammonia, temperature, moisture, humidity, litter pH and feed consumption were recorded daily throughout the experiment. Air and litter microbial counts were recorded on weekly basis. Ammonia, humidity and pH levels depicted significantly (P<0.05) lower level in treatment groups (ZnO @ 500 mg/m²), (ZnO @1000 mg/m²) and (ZnO @ 1500 mg/m²) from the control positive group in the 3^rd week. Air microbial count was significantly (P<0.05) lower in ZnO (@1000 mg/m²) group in 3^rd week from the control positive group. In week 4, all the treatment groups showed significantly (P<0.05) lower litter microbial count from that of the control positive group. The study showed that copper oxide, zinc oxide and alum nanoparticles can be used as possible substitute to overcome the in-house environmental problems. However, there is need to investigate the real impact of these nanoparticles as environmental modifiers.