PAKISTAN VETERINARY JOURNAL

Pak Vet J, 2022, 42(4): 533-539

Molecular Epidemiological Investigation of Cryptosporidium sp., Giardia duodenalis, Enterocytozoon bieneusi and Blastocystis sp. Infection in Free-ranged Yaks and Tibetan Pigs on the Plateau

Xiushuang Chen¹, Nahla Mohammad Saeed², Jinxue Ding¹, Hailong Dong³, Muhammad Fakhar-e-Alam Kulyar⁴, Zeeshan Ahmad Bhutta⁵, Khalid Mehmood^{6, *}, Muhammad Muddassir Ali⁷, Irfan Irshad⁸, Jiangyong Zeng⁹, Jiaguo Liu¹, Qingxia Wu^{3, *} and Kun Li¹^{, *}

¹Institute of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, PR China; ²Department of Microbiology, College of Veterinary Medicine, University of Sulaimania, Kurdistan, Iraq; ³Key laboratory of clinical veterinary medicine in Tibet, Tibet Agriculture and Animal Husbandry College, Linzhi, 860000, Tibet, People's Republic of China; ⁴College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China; ⁵College of Veterinary Medicine, Chungbuk National University, Cheongju, Chungbuk, 28644, Republic of Korea; ⁶Faculty of Veterinary Sciences, The Islamia University of Bahawalpur, 63100, Pakistan; ⁷Institute of Biochemistry and Biotechnology, University of Veterinary and Animal Sciences Lahore 54000, Pakistan; ⁸Pathobiology section, Institute of Continuing Education & Extension, University of Veterinary and Animal Sciences, Lahore, Pakistan; ⁹Institute of Animal Husbandry and Veterinary Medicine, Tibet Academy of Agricultural and Animal Husbandry Sciences, Lhasa 850000, China
*Corresponding author: lk3005@njau.edu.cn (KL); goodwqx@163.com (QXW), khalid.mehmood@iub.edu.pk

Abstract

Intestinal parasites are of great economic importance in livestock. However, scarcity of data has been found about the prevalence of four important intestinal parasites including Cryptosporidium sp., Giardia duodenalis, Enterocytozoon bieneusi and Blastocystis sp. infection in free-ranged yaks and Tibetan pigs during the winter season on the plateau. Fecal samples of yaks (n=40) and Tibetan pigs (n=60) were collected and molecular identification of these parasites was performed through nested PCR amplification. Positive PCR samples were sequenced and further phylogenetic analysis was performed. Results found that the prevalence of Cryptosporidium sp., G. duodenalis, E. bieneusi and Blastocystis sp. was 10.0, 7.5, 12.5, 7.5% in yaks, and 18.3, 0, 56.7, 50.0% in Tibetan pigs, respectively. Co-infections was found between 2.5-7.5% in yaks and 0-26.7% in Tibetan pigs, respectively. The sequenced samples were identified to be Cryptosporidium sp. and was identified as pig genotype II. Findings of this study will provide an insight to the prevention and control for these important parasites on the high plateau.

To Cite This Article: Chen X, Saeed NM, Ding J, Dong H, Kulyar MFEA, Bhutta ZA, Mehmood K, Ali MM, Irshad I, Zeng J, Liu J, Wu Q, Li K, 2022. Molecular epidemiological investigation of Cryptosporidium sp., Giardia duodenalis, Enterocytozoon bieneusi and Blastocystis sp. infection in free-ranged yaks and tibetan pigs on the plateau. Pak Vet J, 42(4): 533-539. http://dx.doi.org/10.29261/pakvetj/2022.060

Abstract

ISSN 0253-8318 (Print)ISSN 2074-7764 (Online)

ISSN 0253-8318 (Print)
ISSN 2074-7764 (Online)