PAKISTAN
VETERINARY
JOURNAL
     
 
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Molecular Epidemiological Investigation of Cryptosporidium sp., Giardia duodenalis, Enterocytozoon bieneusi and Blastocystis sp. Infection in Free-ranged Yaks and Tibetan Pigs on the Plateau
 
Xiushuang Chen1, Nahla Mohammad Saeed2, Jinxue Ding1, Hailong Dong3, Muhammad Fakhar-e-Alam Kulyar4, Zeeshan Ahmad Bhutta5, Khalid Mehmood6, *, Muhammad Muddassir Ali7, Irfan Irshad8, Jiangyong Zeng9, Jiaguo Liu1, Qingxia Wu3, * and Kun Li1, *
 

1Institute of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, PR China; 2Department of Microbiology, College of Veterinary Medicine, University of Sulaimania, Kurdistan, Iraq; 3Key laboratory of clinical veterinary medicine in Tibet, Tibet Agriculture and Animal Husbandry College, Linzhi, 860000, Tibet, People's Republic of China; 4College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China; 5College of Veterinary Medicine, Chungbuk National University, Cheongju, Chungbuk, 28644, Republic of Korea; 6Faculty of Veterinary Sciences, The Islamia University of Bahawalpur, 63100, Pakistan; 7Institute of Biochemistry and Biotechnology, University of Veterinary and Animal Sciences Lahore 54000, Pakistan; 8Pathobiology section, Institute of Continuing Education & Extension, University of Veterinary and Animal Sciences, Lahore, Pakistan; 9Institute of Animal Husbandry and Veterinary Medicine, Tibet Academy of Agricultural and Animal Husbandry Sciences, Lhasa 850000, China
*Corresponding author: lk3005@njau.edu.cn (KL); goodwqx@163.com (QXW), khalid.mehmood@iub.edu.pk

Abstract   

Intestinal parasites are of great economic importance in livestock. However, scarcity of data has been found about the prevalence of four important intestinal parasites including Cryptosporidium sp., Giardia duodenalis, Enterocytozoon bieneusi and Blastocystis sp. infection in free-ranged yaks and Tibetan pigs during the winter season on the plateau. Fecal samples of yaks (n=40) and Tibetan pigs (n=60) were collected and molecular identification of these parasites was performed through nested PCR amplification. Positive PCR samples were sequenced and further phylogenetic analysis was performed. Results found that the prevalence of Cryptosporidium sp., G. duodenalis, E. bieneusi and Blastocystis sp. was 10.0, 7.5, 12.5, 7.5% in yaks, and 18.3, 0, 56.7, 50.0% in Tibetan pigs, respectively. Co-infections was found between 2.5-7.5% in yaks and 0-26.7% in Tibetan pigs, respectively. The sequenced samples were identified to be Cryptosporidium sp. and was identified as pig genotype II. Findings of this study will provide an insight to the prevention and control for these important parasites on the high plateau.

To Cite This Article: Chen X, Saeed NM, Ding J, Dong H, Kulyar MFEA, Bhutta ZA, Mehmood K, Ali MM, Irshad I, Zeng J, Liu J, Wu Q, Li K, 2022. Molecular epidemiological investigation of Cryptosporidium sp., Giardia duodenalis, Enterocytozoon bieneusi and Blastocystis sp. infection in free-ranged yaks and tibetan pigs on the plateau. Pak Vet J, 42(4): 533-539. http://dx.doi.org/10.29261/pakvetj/2022.060  

 
   
 

ISSN 0253-8318 (Print)
ISSN 2074-7764 (Online)



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