Multidrug resistant (MDR) bacteria are creating a serious challenge to treat diseases. The present study was aimed to evaluate the antibacterial and antibiofilm activity of four different essential oils (Melissa officinalis L., Nigella sativa L., Laurus nobilis L., and Origanum onites L.) against MDR bacteria from cats and dogs. Methicillin-resistant Staphylococcus aureus (MRSA), two different multi-drug resistant S. aureus (MDR SA1 and MDR SA2), and Escherichia coli (ESBL E. coli) that contained extended-spectrum beta-lactamases strains were used in the study. Effectiveness of these oils against MDR bacteria was determined through minimum inhibitory concentrations (MIC) test, minimum bactericidal concentrations (MBC) test and antibiofilm assay. Results the inhibitory effect of essential oils determined activity as oregano>melissa>laurel=nigella. Therefore, the most susceptible isolate to essential oils was MRSA. The results also showed significant antibiofilm activity of melissa and oregano against MDR bacteria. In conclusion, the current study declares essential oils from medicinal plants are effective natural products for resistant pathogen bacteria.