Negative Regulation of RpoS-mediated STM1703 in Biofilm Formation of
Salmonella Pullorum
Zheng Feng1,2,3,4, Muhanad El Hag1,2,3,4, Nan
Wang1,2,3,4, Tao Qin1,2,3,4, Sujuan Chen1,2,3,4
and Daxin Peng1,2,3,4*
1College
of Veterinary Medicine, Yangzhou University, Yangzhou 225009,
Jiangsu, PR China
2Jiangsu
Co-Innovation Center for the Prevention and Control of Important
Animal Infectious Diseases and Zoonoses, Yangzhou 225009, Jiangsu,
PR China
3Jiangsu
Research Centre of Engineering and Technology for Prevention and
Control of Poultry Disease, Yangzhou 225009, Jiangsu, PR China
4Joint
Laboratory Safety of International Cooperation of Agriculture and
Agricultural-Products, Ministry of Education, Yangzhou University,
PR China
*Corresponding author:
pengdx@yzu.edu.cn
Abstract
Both alternative sigma factor RpoS and
ubiquitous secondary messenger c-di-GMP
participate
in the biofilm forming of Salmonella
Pullorum; however, the relationship
between RpoS and c-di-GMP-regulated
genes during biofilm forming remains
unclear. In this study, nine genes related with c-di-GMP regulation were
found
to
be
differentially expressed (P<0.01)
by RNA-seq analysis when compared with S.
Pullorum strain S9 and its
rpoS
deletion strain S9S.
Specifically,
the rpoS deletion strain S9S had
higher transcription
level
of
gene STM1703
and lower c-di-GMP concentration and biofilm-forming ability than S9
(P<0.01).
The
STM1703 gene
deletion
in strains S9 and S9S significantly enhanced the c-di-GMP concentration and the
biofilm-forming ability (P<0.01).
qRT–PCR analysis showed that rpoS
deletion or P193L substitution in RpoS increased the transcription level of
the
STM1703 gene by decreasing the transcription levels of
thecsrA and STM1344 genes (P<0.01).
Overall, RpoS-mediated STM1703 negatively regulates the biofilm formation of
S. Pullorum by degrading c-di-GMP.
To Cite This Article:
Feng Z, Hag ME, Wang N, Qin T, Chen S and Peng D, 2022.
Negative regulation of RpoS-mediated STM1703 in biofilm formation of
Salmonella pullorum.
Pak Vet J. http://dx.doi.org/10.29261/pakvetj/2022.086