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Exploring the Molecular Mechanisms of Sophorae tonkinensis Radix et Rhizoma anti-DHAV-1 by Network Pharmacology Analysis
Weiran Wang1 #, Tao Zhang2 #, Muhammad Fahad Iqbal3, Hassan Ashfaq4, Irfan Irshad4, Khalid Mehmood5 $, Kun Li1* and Jiaguo Liu1 *

1MOE Joint International Research Laboratory of Animal Health and Food Safety and Institute of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, P. R. China.
2Beijing Key Laboratory of Traditional Chinese Veterinary Medicine at Beijing University of Agriculture, Beijing 102206, P. R. China; 3Avicenna Medical College, Lahore 54000, Punjab, Pakistan; 4Institute of continuing education and extension, University of Veterinary animal sciences,54000, Lahore Pakistan; 5Faculty of Veterinary and Animal Sciences, The Islamia University of Bahawalpur 63100, Pakistan.
# These authors have contributed equally to this work.
$ Contributed equally to KL and JL.
*Corresponding author: (JGL); (KL)


The study investigated the bioactive ingredients and the anti-duck hepatitis A virus 1 (DHAV-1) mechanisms of Sophorae tonkinensis Radix et Rhizoma (STR) by network pharmacology (NP) and molecular docking (MD). The main bioactive ingredients of the STR were obtained using TCMSP database. Cytoscape 3.8.2 software was used for topology analysis and construction of the STR-active molecule-target interaction network. The STRING database and Cytoscape plotted Protein-protein interaction (PPI) networks. The key targets of STR were analyzed and enriched by Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment. Finally, the main bioactive ingredients of the STR were verified by MD. The STR-DHAV-1 target network included 13 ingredients and 34 target genes. The key target gene is IL-6. KEGG analysis revealed that the main pathways included AGE-RAGE signaling pathway in diabetic complications, pathways in cancer, and C-type lectin receptor signaling pathway. MD results further verified that the main bioactive components identified in the STR were quercetin, kaempferol and matrine, which had higher binding activities to target. Network pharmacology and molecular docking studies revealed that quercetin, kaempferol and matrine were the main bioactive ingredients of STR and might play a crucial role in potential molecular DHAV-1 therapeutic mechanisms.

To Cite This Article: Wang W, Zhang T, Iqbal MF, Ashfaq H, Irshad I, Mehmood K, Li K and Liu J, 2022. Exploring the molecular mechanisms of Sophorae tonkinensis Radix et Rhizoma anti-DHAV-1 by network pharmacology analysis. Pak Vet J.


ISSN 0253-8318 (Print)
ISSN 2074-7764 (Online)