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Age-dependent Cytokine Expression in Response to Foot-and-mouth Disease Virus in Bovine Peripheral Blood Mononuclear Cells
Seung-Won Yi1, Ngoc Anh Bui2, Hu Suk Lee3,4, Vuong Nghia Bui2, Duy Tung Dao2, Trang Huyen Nguyen2, Han Gyu Lee1, Young-Hun Jung1, Tai-Young Hur1 and Sang-Ik Oh1, *

1Division of Animal Diseases & Health, National Institute of Animal Science, Rural Development Administration, 1500 Kongjwipatjwi-ro, Wanju 55365, Republic of Korea; 2Virology Department, National Institute of Veterinary Research, Hanoi, Vietnam; 3International Livestock Research Institute, Hanoi, Vietnam; 4College of Veterinary Medicine, Chungnam National University, Daejeon 34134, Republic of Korea
*Corresponding author:


The severity of foot-and-mouth disease virus (FMDV) infections differs between calves and cattle. Here, we compared immunological responses to FMDV in bovine peripheral blood mononuclear cells (PBMCs) from juvenile cattle (7–9 months) and calves (3–4 months). PBMCs were collected from cattle and inoculated with serotype O FMDV and incubated for 0, 1, 3, 6, 12, 24, 48, 72, and 96 h. At each time point, total RNA was isolated from PBMCs and the mRNA expression of six cytokines (IFN-γ, TNF-α, IL-2, IL-4, IL-6, and IL-10) was evaluated. Th1 (IFN-γ and IL-2) and Th2-related (IL-4 and IL-10) cytokine levels were more prominent in juvenile cattle than in calves. In particular, IL-2 and IL-10 from juvenile cattle were significantly (p < 0.001) higher than those from calves at 48 and 24 hpi, respectively. Therefore, the juvenile cattle showed remarkable Th1 and Th2-related immune response than calves within 48 h after FMDV infection.

To Cite This Article: Yi SW, Bui NA, Lee HS, Bui VN, Dao DT, Nguyen TH, Lee HG, Jung YH, Hur TY and Oh SI, 2022. Age-dependent cytokine expression in response to foot-and-mouth disease virus in bovine peripheral blood mononuclear cells. Pak Vet J.


ISSN 0253-8318 (Print)
ISSN 2074-7764 (Online)