Age-dependent Cytokine Expression in Response to Foot-and-mouth
Disease Virus in Bovine Peripheral Blood Mononuclear Cells
Seung-Won Yi1, Ngoc Anh Bui2, Hu Suk Lee3,4,
Vuong Nghia Bui2, Duy Tung Dao2, Trang Huyen
Nguyen2, Han Gyu Lee1, Young-Hun Jung1,
Tai-Young Hur1 and Sang-Ik Oh1,
*
1Division
of Animal Diseases & Health, National Institute of Animal Science,
Rural Development Administration, 1500 Kongjwipatjwi-ro, Wanju
55365, Republic of Korea; 2Virology Department, National
Institute of Veterinary Research, Hanoi, Vietnam; 3International
Livestock Research Institute, Hanoi, Vietnam; 4College of
Veterinary Medicine, Chungnam National University, Daejeon 34134,
Republic of Korea
*Corresponding author:
ohsangik@korea.kr
Abstract
The severity of foot-and-mouth disease virus (FMDV) infections differs
between
calves and cattle. Here, we compared immunological responses to
FMDV in bovine
peripheral blood mononuclear cells
(PBMCs) from
juvenile cattle (7–9 months) and calves (3–4 months). PBMCs were collected from
cattle and inoculated with serotype O FMDV and incubated for 0, 1, 3, 6, 12, 24,
48, 72, and 96 h.
At each time point, total RNA was isolated from PBMCs and the mRNA expression of
six cytokines (IFN-γ,
TNF-α,
IL-2,
IL-4,
IL-6, and
IL-10) was evaluated. Th1 (IFN-γ
and IL-2) and Th2-related (IL-4
and IL-10) cytokine levels were more
prominent in juvenile cattle than in calves. In particular,
IL-2 and
IL-10 from juvenile cattle were
significantly (p < 0.001) higher than
those from calves at 48 and 24 hpi, respectively. Therefore, the juvenile cattle
showed remarkable Th1 and Th2-related immune response than calves within 48 h
after FMDV infection.
To Cite This Article:
Yi SW, Bui NA, Lee HS, Bui VN, Dao DT, Nguyen TH, Lee HG, Jung YH, Hur TY and Oh
SI, 2022. Age-dependent cytokine expression in response to foot-and-mouth
disease virus in bovine peripheral blood mononuclear cells. Pak Vet J. http://dx.doi.org/10.29261/pakvetj/2023.003