PAKISTAN VETERINARY JOURNAL

Pak Vet J, xxxx, xx(x): xxx-xxx

Age-dependent Cytokine Expression in Response to Foot-and-mouth Disease Virus in Bovine Peripheral Blood Mononuclear Cells

Seung-Won Yi¹, Ngoc Anh Bui², Hu Suk Lee^3,4, Vuong Nghia Bui², Duy Tung Dao², Trang Huyen Nguyen², Han Gyu Lee¹, Young-Hun Jung¹, Tai-Young Hur¹ and Sang-Ik Oh^1,^*

¹Division of Animal Diseases & Health, National Institute of Animal Science, Rural Development Administration, 1500 Kongjwipatjwi-ro, Wanju 55365, Republic of Korea; ²Virology Department, National Institute of Veterinary Research, Hanoi, Vietnam; ³International Livestock Research Institute, Hanoi, Vietnam; ⁴College of Veterinary Medicine, Chungnam National University, Daejeon 34134, Republic of Korea
*Corresponding author: ohsangik@korea.kr

Abstract

The severity of foot-and-mouth disease virus (FMDV) infections differs between calves and cattle. Here, we compared immunological responses to FMDV in bovine peripheral blood mononuclear cells (PBMCs) from juvenile cattle (7–9 months) and calves (3–4 months). PBMCs were collected from cattle and inoculated with serotype O FMDV and incubated for 0, 1, 3, 6, 12, 24, 48, 72, and 96 h. At each time point, total RNA was isolated from PBMCs and the mRNA expression of six cytokines (IFN-γ, TNF-α, IL-2, IL-4, IL-6, and IL-10) was evaluated. Th1 (IFN-γ and IL-2) and Th2-related (IL-4 and IL-10) cytokine levels were more prominent in juvenile cattle than in calves. In particular, IL-2 and IL-10 from juvenile cattle were significantly (p < 0.001) higher than those from calves at 48 and 24 hpi, respectively. Therefore, the juvenile cattle showed remarkable Th1 and Th2-related immune response than calves within 48 h after FMDV infection.

To Cite This Article: Yi SW, Bui NA, Lee HS, Bui VN, Dao DT, Nguyen TH, Lee HG, Jung YH, Hur TY and Oh SI, 2022. Age-dependent cytokine expression in response to foot-and-mouth disease virus in bovine peripheral blood mononuclear cells. Pak Vet J. http://dx.doi.org/10.29261/pakvetj/2023.003

Abstract

ISSN 0253-8318 (Print)ISSN 2074-7764 (Online)

ISSN 0253-8318 (Print)
ISSN 2074-7764 (Online)