Azadirachta indica (Neem) is an important medicinal plant containing diverse antimicrobial and antiparasitic activities. In the current scenario of emerging drug resistance, there is need of plant derived compounds having antiparasitic activity to counter infectious problem including helminths. Present research was conducted to evaluate the phytochemical composition, ovicidal and adulticidal activity of methanolic and ethyl acetate extracts of Azadirachta indica (Neem). The leaves of Neem were used for preparation of extracts and their composition was determined through high performance liquid chromatography (HPLC). Results showed that the methanolic extract had higher quercetin compounds than the ethyl acetate extract. The effect of these extracts on egg hatch and larval motility were tested in in vitro experiment. The results indicated that these were found effective to reduce the hatchability of eggs. Hatching percentage was 1.12% ± 1.01 in case of methanolic extract and 3.57% ± 0.97 in case of ethyl acetate extract. Ethyl acetate extract of A. indica killed all the worms at the concentration of 25 mg/mL at 6 hrs post exposure while methanolic extract showed 100 percent mortality at 10 hrs post exposure of same dose. Results were in dose-dependent manner. Ethyl acetate extract killed adult worms faster and at lower dose than methanolic extract. However, the methanolic extract of Neem had higher effectiveness than the ethyl acetate extract in preventing hatching of eggs. This research concludes that extraction solvent greatly affects the phytochemical composition and anthelmintic activities of Neem and this is effective to control the helminths.