PAKISTAN
VETERINARY
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Antiparasitic Activity of Methanolic and Ethyl acetate Extracts of Azadirachta indica against Haemonchus contortus
 
Tauseef ur Rehman1*, Ahmed A. El-Mansi2,3, Sadeq K. Alhag4, Laila A. Al-Shuraym5, Zohaib Saeed6, Muhammad Arif7, Muhammad Rashid1, Zeeshan Ahmad Bhutta8 and Muhammad Arfan Zaman9
 
1Department of Parasitology, The Islamia University of Bahawalpur, Pakistan; 2Biology Department, Faculty of Science, King Khalid University, Abha, Saudi Arabia; 3Zoology Department, Faculty of Science, Mansoura University, Mansoura, Egypt; 4Biology Department, College of Science and Arts, King Khalid University, Muhayl Asser, Saudi Arabia; 5Biology Department, Faculty of Science, Princess Nourah Bint Abdulrahman University, Riyadh, Saudi Arabia; 6Department of Parasitology, University of Agriculture Faisalabad, 38040, Pakistan; 7Veterinary Officer, Livestock production research institute, Bahadurnagar, Okara, Pakistan; 8Laboratory of Biochemistry and Immunology, College of Veterinary Medicine, Chungbuk National University, Cheongju, Chungbuk, Republic of Korea; 9Department of Parasitology, College of Veterinary and Animal Sciences, Jhang, Pakistan
*Corresponding author: drtauseef@iub.edu.pk

Abstract   

Azadirachta indica (Neem) is an important medicinal plant containing diverse antimicrobial and antiparasitic activities. In the current scenario of emerging drug resistance, there is need of plant derived compounds having antiparasitic activity to counter infectious problem including helminths. Present research was conducted to evaluate the phytochemical composition, ovicidal and adulticidal activity of methanolic and ethyl acetate extracts of Azadirachta indica (Neem). The leaves of Neem were used for preparation of extracts and their composition was determined through high performance liquid chromatography (HPLC). Results showed that the methanolic extract had higher quercetin compounds than the ethyl acetate extract. The effect of these extracts on egg hatch and larval motility were tested in in vitro experiment. The results indicated that these were found effective to reduce the hatchability of eggs. Hatching percentage was 1.12% ± 1.01 in case of methanolic extract and 3.57% ± 0.97 in case of ethyl acetate extract. Ethyl acetate extract of A. indica killed all the worms at the concentration of 25 mg/mL at 6 hrs post exposure while methanolic extract showed 100 percent mortality at 10 hrs post exposure of same dose. Results were in dose-dependent manner. Ethyl acetate extract killed adult worms faster and at lower dose than methanolic extract. However, the methanolic extract of Neem had higher effectiveness than the ethyl acetate extract in preventing hatching of eggs. This research concludes that extraction solvent greatly affects the phytochemical composition and anthelmintic activities of Neem and this is effective to control the helminths.

To Cite This Article: Rehman TU, El-Mansi AA, Alhag SK, Al-Shuraym LA, Saeed Z, Arif M, Rashid M, Bhutta ZA and Zaman MA, 2023. Antiparasitic activity of methanolic and ethyl acetate extracts of Azadirachta indica against Haemonchus contortus. 43(1): 199-203. http://dx.doi.org/10.29261/pakvetj/2023.014

 
   
 

ISSN 0253-8318 (Print)
ISSN 2074-7764 (Online)



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