The present study was intended to ascertain the attenuative role of rhamnetin (RHM) against polystyrene microplastics (PS-MPs) prompted nephrotoxicity in rats. 24 male albino rats (6-8 weeks old) were arbitrarily separated into 4 groups viz. control, PS-MPS (0.01mg/kg) treated group, RHM+PS-MPs (0.01mg/kg + 50mg/kg) co-treated group and only RHM (50mg/kg) treated group. The experiment was executed for 30 days. At the last day, all the rats were slaughtered to analyze the various kidney parameters. Our findings demonstrated that PS-MPs exposure significantly lowered the enzymatic antioxidant activities (CAT, GPx, GSR, SOD, GST & GSH), while it augmented the level of oxidative stress markers (ROS and MDA). PS-MPs administration significantly up-regulated the level of KIM-1, NGAL, serum urea & creatinine, whereas reduced the creatinine clearance. Additionally, PS-MPs significantly elevated the inflammatory markers level such as TNF-α (tumor necrosis factor alpha), IL-1β (Interleukin-1 beta), NF-κB (nuclear factor kappa-B), IL-6 (Interleukin-6) and COX-2 (cyclooxygenase-2) activity along with histological abnormalities. However, RHM co-treatment with PS-MPs exhibited protective effect against PS-MPs provoked nephrotoxicity and restored all the anomalies. These findings have proposed that RHM is a potent flavone that can mitigate the kidney damages caused by PS-MPs.