Canine parvovirus (CPV) is a highly contagious viral disease and the most significant intestinal pathogens affecting dogs as well as puppies, making these infections a real danger on dog population worldwide. Therefore, this study was to elucidate and detect canine parvovirus strains circulating in Palestine. This was achieved by molecular analyzing of VP2 gene by polymerase chain reaction (PCR). In the current study, a total number of 25 dogs suffered from severe watery bloody diarrhea, vomiting and lethargy were examined serologically to confirm parvovirus antigens. Complete Blood count (CBC) was also involved to assess the effects of the virus on the hematological parameters of each dog. The PCR positive samples were evaluated by Sanger’s sequencing method to characterize the virus and to obtain the essential information about the genotypes and nucleotide polymorphisms of CPV strains circulating in Palestine. The partial nucleotide sequences of VP2 gene were compared with reference VP2 gene sequences of CPV recorded in GenBank database. The phylogenetic analysis revealed that 24/25 (96%) of the sequences belonged to serotype CPV-2c and 1/25 (4%) belonged to serotype CPV-2b. The current obtained sequences were registered at the GenBank database under the following accession numbers: OQ924950- OQ924974. To our knowledge, this report is considered the first one to investigate the molecular characterization of CPV-2 in Palestine. This finding could be useful for commercial vaccine companies to select the suitable strains of CPV that include the prevalent antigenic types of the field virus, to enhance the immunity against CPV in dogs.