The current study was planned to investigate the effects of supplementation of different concentrations of egg yolk (EY) and Carboxylated Poly-l Lysine (CPLL) on the post-thawed sperm quality of male Beetal goats. For this purpose, pooled semen of bucks (n=6) was diluted with Tris-citric acid egg yolk (TCEY) extender containing different concentrations of EY (0, 5 and 15%) and CPLL (0, 0.1, 0.15, 0.20 and 0.25%). Data were presented as means with pooled SEM. The main effects, EY and CPLL concentrations, and their interaction were analyzed using multifactor ANOVA. Minimum values of progressive motility (PM) and motion kinematics of spermatozoa were observed in extenders having 0% EY with different CPLL concentrations. The extender containing 5% EY and 0.25% CPLL resulted in better (P<0.05) PM than 0 and 15% EY extenders with different CPLL concentrations. The ratio (P<0.05) of sperm with intact plasma membrane was higher in 5% EY extender with 0.20 and 0.25% CPLL than in 15% EY extender with corresponding CPLL concentrations The EY concentration significantly influenced sperm acrosomal integrity, as 0% EY extenders showed lower (P<0.05) sperm acrosome integrity than 5 and 15% EY extenders. At various concentrations of CPLL, both 5 and 15% EY extenders had similar (P>0.05) ratios of acrosomal membrane-intact sperm. In comparison to 0 and 15% EY extenders with various CPLL concentrations, 5% EY extenders had better (P<0.05) sperm mitochondrial integrity (MI), especially with 0.25% CPLL concentration. In conclusion, PM, PMI, and MI of Beetal goat spermatozoa were significantly improved in Tris-citrate extender containing 5% EY and supplemented with 0.25% CPLL..