A frequent and efficient weapon in the fight against infectious diseases is antibiotics. Antimicrobial resistance, however, has emerged as a major concern in public health due to the unintentional, overuse, and improper administration of antibiotics. In the current study, a heterostructure of gold (Au), Hydroxyapatite (HAP) and Cefuroxime was produced using an environmentally friendly pulsed laser ablation technique to improve the antibacterial activity of Cefuroxime against Staphylococcus (S.) saprophyticus. X-ray diffraction (XRD), Fourier transforms infrared spectroscopy, UV/Vis spectrophotometry, and field emission-scanning electron microscopy (FE-SEM) were used to characterize the heterostructure nanocomposite. The susceptibility of S. saprophyticus to the Au-Hydroxyapatite-Cefuroxime was evaluated in terms of preventing biofilm development in the urinary catheter. This study confirmed the greater antimicrobial activity of hetero nanocomposite of the Hydroxyapatite-Cefuroxime-AuNPs than Cefuroxime alone against S. saprophyticus. The Hydroxyapatite nanoparticles (NPs)-Cefuroxime-Gold nanoparticles (AuNPs) hybrid was developed as an intelligent drug delivery mechanism to prevent the proliferation of S. saprophyticus and to hinder the formation of bacterial biofilm on Foley catheters. The smart hetero nanocomposite has shown a promising result, which could be a reliable therapy against S. saprophyticus in the future and could be used as a promising approach for succeeding bacterial biofilm inhibition during urinary tract infection.