The avian orthoavulavirus type 1, previously recognized as avian paramyxovirus 1, is responsible for the widely recognized Newcastle disease virus (NDV) within the poultry field. This disease has substantial and adverse effects on crucial avian systems, including the respiratory, gastrointestinal, and reproductive systems. The aim of our study was to develop a high-resolution melting (HRM) assay targeting the fusion (F) gene, aimed at discriminating between NDV genotype VII and its associated subgenotypes. Then HRM results were compared with those obtained through sequencing of the F gene. The primers were specifically designed to suit NDV genotype VII and its corresponding subgenotypes (1.1, 1.2, 2, and 1.1L). Subsequently, these primers, underwent thorough assessment using 14 out of 24 clinical samples and positive controls. Notably, all assessed clinical samples exhibited a genotype VII profile in the HRM assay, characterized by a melting temperature of 77.95 ± 0.04°C. Subgenotype 1.1 manifested a distinctive melting temperature of 82.41± 0.02°C, while subgenotype 1.2 displayed 81.8 ± 0.02°C, subgenotype 2 exhibited 80.28 ± 0.02°C, and subgenotype 1.1l indicated 79.39 ± 0.06°C. Remarkably, the sequencing results from all 14 samples were in concordance with the HRM outcomes. These developed HRM assays were able to differentiate the NDV genotype VII and its subgenotypes thus providing alternative for genotyping of NDV sequencing of F gene.