Molecular Characterization of Important Viruses
Contributing to Bovine Respiratory Disease Complex in Türkiye
Remziye Ozbek1*, Hasan Abayli2, Sükrü Tonbak2
and Kezban Sahna2
1Department
of Virology, Faculty of Veterinary Medicine, Sivas Cumhuriyet
University, Sivas, Türkiye; 2Department of Virology,
Faculty of Veterinary Medicine, Firat University, Elazig, Türkiye
*Corresponding author:
remziyeozbek@cumhuriyet.edu.tr
Abstract
Bovine respiratory disease complex (BRDC) is one of the most common cattle
diseases, causing widespread effect in worldwide. That pathogens causing BRDC
occur with coinfections may even lead irreversible losses. That is why, in this
study, it is aimed to investigate crucial viral pathogens associated with BRDC
[Bovine herpesvirus-1 (BoHV-1); bovine respiratory syncytial virus (BRSV),
bovine viral diarrhoea virus (BVDV), Hobi-like pestivirus, bovine
parainfluenza-3 virus (BPIV-3)] in the same sample simultaneously. Aligned with
the aim, 129 nasal swabs were collected from cattle with clinical symptoms of
respiratory system disease in specific provinces of eastern and southeastern
Türkiye. Firstly, viral RNA and DNA were isolated from the samples.
Subsequently, PCR and RT-PCR analyses were conducted using specific primers for
the viral pathogens. The results illustrated 10.85 BRSV, 1.55 BVDV-1 and 3.8%
BPIV-3 positivity rates. BoHV-1, Hobi-like pestivirus, and mixed infection were
not detected in any of the samples. Samples that tested positive were sequenced
to represent each province for molecular characterisation and phylogenetic
analysis. They were determined that the BRSV strains (TR/Bngol-04/BRSV,
TR/Dyrbkr-17/BRSV, and TR/Elz-23/BRSV) obtained from this study are a part of
subgroup III, BVDV strains (BVDV-1/TR-/Malatya-1 and BVDV-1/TR/Elazig-29)
belonged to subgroup BVDV 1l, and the BPIV-3 strains (BPIV3/M17/TUR and
BPIV3/M19/TUR) were within genotype C. The findings of these studies can be
valuable to minimise the losses attributed to BRDC by facilitating the
development of diagnostic, molecular, and epidemiological approaches. These
insights can also serve as a foundation for establishing targets for vaccine
development, ultimately contributing to the reduction of BRDC.
To Cite This Article:
Ozbek R, Abayli H, Tonbak S and Sahna K, 2024.
Molecular characterization of important viruses contributing to bovine
respiratory disease complex in Türkiye. Pak Vet J, 44(2): 322-329. http://dx.doi.org/10.29261/pakvetj/2024.155