Detection and Molecular
Characterization of Canine and Feline Bocaparvoviruses in Türkiye
Zeynep Karapinar1, * and Mehmet Ozkan Timurkan2
1Department
of Virology, Faculty of Veterinary Medicine, Balikesir University,
Balikesir, Türkiye; 2Department of Virology, Faculty of
Veterinary Medicine, Atatürk University, Erzurum, Türkiye
*Corresponding author:zeynepkarapinar@gmail.com
Abstract
The genus Bocaparvovirus (BoV) in the subfamily Parvovirinae shows a wide
host spectrum and causes infection with different clinical findings in various
animals. In recent years, canine and feline Bocaparvovirus infections, which are
novel parvovirus species, have been reported in different geographical regions.
The aim of this study is to investigate the presence of canine and feline
Bocaparvovirus genotypes in dog and cat fecal samples and to perform their
molecular characterization. The presence of canine and feline bocaparvovirus
nucleic acid was investigated in stool samples taken from 74 dogs and cats.
After PCR tests, it was found that five out of 46 dogs were positive for CBoV-1
and five out of 46 dogs were positive for CBoV-2, while two out of 28 cats were
positive for FBoV-2. The sequence comparison of the capsid protein gene (VP2) of
the five CBoV-1 identified in this study shared a high identity with each other
(96.4-99.2% nt and 94.6–99.2% aa identities) and with previously reported CBoV-1
strains (93.4-98.9% nt and 93.9–100% aa identities). It was determined that five
CBoV-1 strains were located in Lineage 2 in the phylogenetic map. The nucleotide
and amino acid comparison showed that the non-structural protein (NS1) gene
sequences of the five CBoV-2 strains identified in this study shared a high
identity with each other (93.5-97.0% nt and 93.6–100% aa identities) and with
previously reported CBoV-2s (92.2–98.6 % nt and 92.8–100% aa identities). The
phylogenetic map showed three strains (OR690113, OR690112, OR690117) in Lineage
1 and two strains (OR690114, OR690115) in Lineage 2. The sequence comparison of
the nuclear phosphoprotein-1 (NP1) gene sequences of the two
FeBoV strains identified in this study shared
identity with each other (100% nt and aa identities) and with previously
reported FeBoV strains (63.8-99.5% nt and 59.4–100% aa identities). As a result
of the sequence analysis, it was determined that the two FBoV-2 strains
identified in the study were included in Lineage 1. Findings of the present
study compile current genetic data and contribute to the molecular epidemiology
of these infections. It is recommended to evaluate Bocaparvoviruses, use
molecular methods and conduct further studies on the diagnosis of infections in
dogs and cats.
To Cite This Article:
Karapinar Z and Timurkan MO, 2024.
Detection and molecular characterization of canine and feline
bocaparvoviruses in Türkiye. Pak Vet J, 44(2): 361-368.
http://dx.doi.org/10.29261/pakvetj/2024.145