Could Paenibacillus xylanexedens MS58 be
an Ecofriendly Antibiotic in Poultry Production? Impacts on
Performance, Blood Biochemistry, Gut Microbiota and Meat Quality
Anas S. Dablool1, Banan Atwah2,
Saad Alghamdi2, Maha Abdullah Momenah3,Ohud Saleh4,
Nada Alhazmi5,6, Yasser S. Mostafa7, Saad A.
Alamri7, Worood A.A. Alyoubi8, Naheda M.
Alshammari9, Alaa S. Mohamed10,
Nadeen G. Mostafa11, and Belal A. Omar12*
1Public
Health Department, Health Sciences College at Al-Leith, Umm Al-Qura
University, Makkah, Saudi Arabia; 2Department of Clinical
Laboratory Sciences, Faculty of Applied Medical Sciences, Umm Al-Qura
University, Makkah, Saudi Arabia; 3Department of Biology,
College of Science, Princess Nourah bint Abdulrahman University,
P.O. Box 84428, Riyadh 11671, Saudi Arabia; 4Department
of Biochemistry, College of Science, University of Jeddah, Jeddah
21959, Saudi Arabia; 5Department of Basic Sciences,
College of Science and Health Professions, King Saud bin Abdulaziz
University for Health Sciences, Jeddah 11481, Saudi Arabia; 6King
Abdullah International Medical Research Center, Jeddah, Saudi
Arabia; 7Department of Biology, College of Science, King
Khalid University, Abha P.O. Box 9004, Saudi Arabia; 8Biological
Sciences Department, College of Science & Arts, King Abdulaziz
University, Rabigh 21911, Saudi Arabia; 9Department of
Biological Sciences, Microbiology, Faculty of Science, King
Abdulaziz University, Jeddah 21589, Saudi Arabia; 10Food
Science Department, Faculty of Agriculture, Zagazig University,
Zagazig 44511, Egypt; 11Department of Agricultural
Microbiology, Faculty of Agriculture, Zagazig University, Zagazig
44511, Egypt; 12Biochemistry Department, Faculty of
Agriculture, Zagazig University, Zagazig 44511, Egypt *Corresponding author:Belalaomar@gmail.com (Belal A. Omar)
Abstract
The search for novel
natural antibiotic alternatives is continuous and crucial to overcome the
antibiotic-resistant bacteria. In this study, the isolation and identification
of Paenibacillus
xylanexedens MS58(PX)
on the gene level as a new probiotic, antibiotic alternative, and feed
additive has been assessed. The effects of PX on growth performance, blood
biochemical parameters, immune response, gut microbiota and meat quality of
broilers were also evaluated. The selected isolate, Paenibacillus xylanexedens MS58,inhibited the growth of pathogenic bacteria with MIC of
5-15 %. One hundred sixty
chicks were casually divided into four equal experimental groups: the control
group (CON) delivered the basal diet, group 1 received a basal diet supplemented
with 0.3 mg/kg PX, and groups 2 and 3 provided a basal diet fortified with PX at
0.9 and 1.5 mg/kg, respectively. Adding PX at 1.5 mg/kg to the diet of chicks
significantly enhanced the highest body weight influenced all growth parameters
during 35 days (2.304 kg) with a relative increase of 10 % compared to control.
Additionally, the dietary PX enhanced the liver and kidney enzymes; ALT activity
decreased by 25% and AST by 67% compared to control. The uric acid was lowered
by dietary PX with a relative decrease of 18 %. In response to adding dietary
PX, the abdominal fat reduced from 1.1 to 0.8 in the PX 1.5 treatment with a
relative reduction of 25 %, and total cholesterol decreased by 33 %; however,
HDL increased. Additionally, the immune response and beneficial gut microbiota
of chicks significantly improved with the addition of the dietary PX compared to
the control group. The chicken meat's moisture and protein content were
enhanced, influenced by the juiciness and tenderness properties compared to
control; also, the lightness of meat was enhanced with PX addition. Generally,
adding dietary PX to broilers could enhance growth, blood biochemical
properties, and immune response, modulate the gut microflora structure, and
improve meat quality.
To Cite This Article:
Dablool AS, Atwah B, Alghamdi S, Momenah MA,
Saleh O, Alhazmi N, Mostafa YS, Alamri SA, Alyoubi WAA, Alshammari NM,Mohamed AS,Mostafa NG, and Omar BA, 2024.
Could Paenibacillus xylanexedens MS58 be an ecofriendly antibiotic in
poultry production? impacts on performance, blood biochemistry, gut microbiota
and meat quality. Pak Vet J, 44(2): 352-360. http://dx.doi.org/10.29261/pakvetj/2024.180