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Insights into NDV Distribution and Molecular Detection Across Multiple Regions of Khyber Pakhtunkhwa Province, Pakistan
Muhammad Tariq Zeb1,2, Irshad Ahmad1*, Muhammad Tahir Khan3,4,5*, Muhammad Tahir Sarwar1 and Nighat Nawaz5

1Institute of Basic Medical Sciences, Khyber Medical University, Peshawar, Pakistan; 2Genomic Laboratory, Veterinary Research Institute, Peshawar, Pakistan; 3Institute of Molecular Biology & Biotechnology (IMBB), the University of Lahore, K.M. Defence Road, Lahore, Pakistan; 4Zhongjing Research & Industrialization Institute of Chinese Medicine, Zhongguancun Scientific Park, Meixi, Nanyang, Henan, 473006, P.R. China; 5INTI International University, Persiaran Perdana BBN Putra Nilai, 71800 Nilai, Negeri Sembilan, Malaysia; 6Lecturer, Department of Chemistry, Islamia College Peshawar
*Corresponding author: (IA); (MTK)


The study investigated Newcastle Disease Virus (NDV) distribution across different regions of Pakistan's Khyber Pakhtunkhwa province. A total of 400 tissue samples, i-e, proventriculus, liver, lungs, trachea (dead birds), and oropharyngeal, cloaca swabs (live birds) were collected from Peshawar, Hazara, Southern, and Malakand, revealing varied NDV positivity rates. Malakand had the highest rate (25.7%), followed by Peshawar and Hazara (20%), and the Southern region (17.8%). Statistical analysis indicated potential significant differences in NDV prevalence among regions (P-value = 0.031, Fisher's exact test). Among the 400 samples, 80 underwent RNA extraction and cDNA synthesis. PCR amplification of the F gene showed successful results in 62 samples, indicating mesogenic and velogenic strains. Further assays categorized 34 samples as velogenic/mesogenic, 20 as lentogenic, and 28 as mixed strains. Among 32 positive velogenic/mesogenic samples, 25 remained undigested with the BglI enzyme, confirming their velogenic nature due to the presence of the fusion protein cleavage site. The current study shows NDV's regional distribution, revealing their diversity in the KP Province of Pakistan. These findings are useful for better management of NDV in the future, offering insights for potential control measures against this infectious disease.

To Cite This Article: Zeb MT, Ahmad I, Khan MT, Sarwar MT and Nawaz N, 2024. Insights into NDV Distribution and Molecular Detection Across Multiple Regions of Khyber Pakhtunkhwa Province, Pakistan. Pak Vet J.


ISSN 0253-8318 (Print)
ISSN 2074-7764 (Online)