Acrylamide (ACR) is created when foods high in carbohydrates are heated up and is more harmful because it produces ROS inside cells. Our research aimed to investigate the potential of coenzyme Q10 (CoQ10) and rosuvastatin (ROSV) in mitigating the adverse effects of testicular toxicity induced by ACR in rats. A total of 49 male rats were distributed among 07 groups. Group 1 served as the control and was administered normal saline orally, while Group 2 received oral CoQ10 at a dosage of 10 mg/kg BW; Group 3, was administered ROSV (10 mg/kg/BW, orally); group 4, received ACR orally (20 mg/kg/BW); group 5 received ACR and CoQ10; group 6 administered ACR and ROSV; and group 7, the rats received ACR, CoQ10, and ROSV daily for 30 days. Acrylamide lowered serum testosterone, FSH, and LH levels, and marked elevations in testicular MDA levels were observed alongside notable decreases in GSH, CAT and SOD. It caused a significant reduction in the diameter of seminiferous tubules, numbers of spermatocytes, spermatogonia, Sertoli cells, germinal epithelium height, and a dramatic increase in lumen diameter. Also, the testicular histopathology was disturbed by ACR, and the testicular caspase-3 expression was considerably elevated in the ACR-treated group. In rats exposed to ACR intoxication, CoQ10 and ROSV raised levels of antioxidant biomarkers, lowered MDA and mitigated alterations in serum hormonal parameters. In summary, our results suggest that CoQ10 and ROSV exhibit considerable protective effects against ACR-induced testicular toxicity, attributed to their anti-oxidative properties.