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Testicular Injury of Acrylamide in Rats and Potential Protection of Coenzyme Q10 and Rosuvastatin
Asmaa Elsayed1, Mohamed Aboubakr1*, Fatma Wahba Hassan2, Marwa Zakaria3, Ehab Yahya Abdelhiee4, Ahmed Soliman5, Anwar El-Shafey6,7, Ahmed Farag8, Ahmed Elfadadny9 and Nehal Abdelmageed1

1Department of Pharmacology, Faculty of Veterinary Medicine, Benha University, 13736 Moshtohor, Toukh, Qaliobiya, Egypt; 2Physiology Department, Faculty of Veterinary Medicine, Cairo University, 12211 Giza, Egypt; 3Department of Pharmaceutical Production Technology, Faculty of Applied Health Science Technology, October Technological University, Giza, Egypt; 4Forensic Medicine and Toxicology Department, Faculty of Veterinary Medicine, Matrouh University, Matrouh, Egypt; 5Department of Pharmacology, Faculty of Veterinary Medicine, Cairo University, 12211 Giza, Egypt; 6Department of Anatomy and Embryology, Faculty of Veterinary Medicine, Benha University, Egypt; 7Department of Basic Veterinary Sciences, Delta University for Science and Technology, Egypt; 8Department of Surgery, Anesthesiology and Radiology, Faculty of Veterinary Medicine, Zagazig University, Zagazig, Egypt; 9Department of Animal Internal Medicine, Faculty of Veterinary Medicine, Damanhur University, Damanhur, Egypt
*Corresponding author:


Acrylamide (ACR) is created when foods high in carbohydrates are heated up and is more harmful because it produces ROS inside cells. Our research aimed to investigate the potential of coenzyme Q10 (CoQ10) and rosuvastatin (ROSV) in mitigating the adverse effects of testicular toxicity induced by ACR in rats. A total of 49 male rats were distributed among 07 groups. Group 1 served as the control and was administered normal saline orally, while Group 2 received oral CoQ10 at a dosage of 10 mg/kg BW; Group 3, was administered ROSV (10 mg/kg/BW, orally); group 4, received ACR orally (20 mg/kg/BW); group 5 received ACR and CoQ10; group 6 administered ACR and ROSV; and group 7, the rats received ACR, CoQ10, and ROSV daily for 30 days. Acrylamide lowered serum testosterone, FSH, and LH levels, and marked elevations in testicular MDA levels were observed alongside notable decreases in GSH, CAT and SOD. It caused a significant reduction in the diameter of seminiferous tubules, numbers of spermatocytes, spermatogonia, Sertoli cells, germinal epithelium height, and a dramatic increase in lumen diameter. Also, the testicular histopathology was disturbed by ACR, and the testicular caspase-3 expression was considerably elevated in the ACR-treated group. In rats exposed to ACR intoxication, CoQ10 and ROSV raised levels of antioxidant biomarkers, lowered MDA and mitigated alterations in serum hormonal parameters. In summary, our results suggest that CoQ10 and ROSV exhibit considerable protective effects against ACR-induced testicular toxicity, attributed to their anti-oxidative properties.

To Cite This Article: Elsayed A, Aboubakr M, Hassan FW, Zakaria M, Abdelhiee EY, Soliman A, El-Shafey A, Farag A, Elfadadny A and Abdelmageed N, 2024. Testicular injury of acrylamide in rats and potential protection of coenzyme Q10 and rosuvastatin. Pak Vet J.


ISSN 0253-8318 (Print)
ISSN 2074-7764 (Online)