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Expression of Pyruvate Dehydrogenase Kinase (PDK) in Lungs during Progression of Pulmonary Hypertension Syndrome in Broilers
Xiaojuan Huang1, Xiaoqin Liu1, Wanli Xu2, Yuanliang Li1, Ping Wu1, Xuqing He1, Dongyang Zhong1, Farid S Ataya3 and Ying Li1*

1College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China; 2Bijie Forage and Feed Station, Bijie, China; 3Department of Biochemistry, College of Science, King Saud University, PO Box 2455, Riyadh 11451, Saudi Arabia
*Corresponding author:


Pulmonary hypertension syndrome (PHS) has always been of significant importance related to cardiovascular diseases. The PHS is one of the major reasons for the high morbidity and mortality in broilers negatively impacting the broiler farming industry. In this study samples from lungs and blood of broilers with enlarged and normal abdomen were collected and fluorescence quantification, immunohistochemistry and histopathology of the selected organs were done to diagnose this disease in broilers. Clinically, it was observed that morbid broilers were depressed, dyspneic, right heart hypertrophied, right ventricle to total ventricle volume (RV/TV) was greater than 0.25 and abdomen was enlarged having yellowish fluid. The diagnosis of PHS was confirmed by the RV/TV and clinical symptoms whereas, the histopathological observations revealed that with age the pulmonary vascular density decreased, lumen of the pulmonary vessels was narrowed and the middle layer of pulmonary vessels was thickened. Hematoxylin and Eosin (HE) staining of the selected samples was done. It was found that age increased in greater interstitial spaces in the lungs of PHS broilers along with inflammatory cells infiltrating the lungs. Results of fluorescence quantification and immunohistochemistry revealed that all types of PDK (PDK1, PDK2, PDK3, PDK4) exhibited significant upregulation in the lungs of broilers with PHS onset and PDK4 was significantly expressed on pulmonary vessels. Therefore, it was concluded that PHS leads to remodeling of the pulmonary vasculature leading to ascites and PDK plays an important role in the progression of PHS.

To Cite This Article: Huang X, Liu X, Xu W, Li Y, Wu P, He X, Zhong D, Ataya FS and Li Y, 2024. Expression of pyruvate dehydrogenase kinase (PDK) in lungs during progression of pulmonary hypertension syndrome in broilers. Pak Vet J.


ISSN 0253-8318 (Print)
ISSN 2074-7764 (Online)

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