PAKISTAN
VETERINARY
JOURNAL
     
 
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Silymarin Antibacterial Efficacy against some Isolated Bacterial Strains from Pneumonic Sheep - Vitro Study
 
Hany Hassan1*, Ahmed Kamr1, Walid Mousa2, Ramiro Toribio3, Abdel Nasser El-Gendy4, Hadeer Khaled1, Abdelsalam Elkholey1 and Ali Arbaga1
 

1Department of Medicine and Infectious Diseases (Animal Internal Medicine), Faculty of Veterinary Medicine, University of Sadat City, Sadat City 32897, Egypt; 2Department of Medicine and Infectious Diseases (Animal Infectious Diseases), Faculty of Veterinary Medicine, University of Sadat City, Sadat City 32897, Egypt; 3College of Veterinary Medicine, the Ohio State University, Columbus, OH 43210, USA; 4Medicinal and Aromatic Plants Research Department, Pharmaceutical and Drugs Research Institute, National Research Centre, Cairo 12622, Egypt
*Corresponding author: hanyhassan1959@gmail.com

Abstract   

Pneumonia is a common disease in sheep flocks that causes severe economic losses and high mortality rate among sheep due to high resistance to ordinary standard antimicrobial treatment protocols. This study was to assess silymarin efficacy as an antibacterial agent against some isolated bacterial strains from twenty-five pneumonic sheep in vitro. Nasopharyngeal swabs were collected from sick sheep, and placed in a nutrient and pleuro pneumonia like organism broth (PPLO) for bacterial isolation. P. aeruginosa, S. aureus, E. coli, and M. ovipeumoniae were isolated bacterial species confirmed by PCR using specific genes. The antibacterial activity of silymarin against P. aeruginosa, S. aureus, and E. coli was evaluated using the well-diffusion technique. It was shown that the minimum therapeutic dosage of silymarin was not less than 280 mg/ml against P. aeruginosa, whereas chloramphenicol had little effect. Chloramphenicol exhibited more antibacterial activity against S. aureus and, E. coli compared to different concentrations of silymarin. The microbroth dilution method determined the minimum inhibitory concentration (MIC) of silymarin against P. aeruginosa, S. aureus, E. coli, and M. ovipeumoniae were 2.14, 0.39, 0.38, and 2.5 mg/ml, respectively. The Minimum Bactericidal Concentrations (MBC) of silymarin were verified by the absence of bacterial growth of the isolated strains that were scattered from the lowest MIC. In conclusion, silymarin exhibited antibacterial efficacy against isolated P. aeruginosa, S. aureus, E. coli, and M. ovipeumoniae from pneumonic sheep in vitro compared to chloramphenicol, suggesting its therapeutic value in sheep.

To Cite This Article: Hassan H, Kamr A, Mousa W, Toribio R, El-Gendy AN, Khaled H, Elkholey A and Arbaga A, 2024. Silymarin antibacterial efficacy against some isolated bacterial strains from pneumonic sheep - vitro study. Pak Vet J, 44(2): 280-285. http://dx.doi.org/10.29261/pakvetj/2024.157

 
   
 

ISSN 0253-8318 (Print)
ISSN 2074-7764 (Online)



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