previous page   Pak Vet J, xxxx, xx(x): xxx   next page
Designing an Epitope-Based Vaccine against Bovine Viral Diarrhea using Immuno-informatics
Rimsha Riaz1, Saira Zahid1 and Muhammad Sarwar Khan1*

Centre of Agricultural Biochemistry and Biotechnology (CABB), University of Agriculture Faisalabad, Pakistan. P. O. Box 38000
*Corresponding author:


Bovine Viral Diarrhea (BVD) is a major economic threat to the livestock industry due to its widespread infection among cattle around the world. Due to resistant strains and regulatory system inadequacies, traditional therapeutic vaccines failed to eradicate it. In this study, a Multi-Epitope Vaccine (MEV) with noticeable immunological properties is proposed that can mitigate these problems by decreasing cross-contamination, autoimmune responses, and inflammatory responses. Using Immunoinformatics approaches, putative CTL- and B-cell epitopes from four BVDV proteins (E1, E2, Erns, and NS3) were predicted and scrutinized after immunogenic profiling. The robust binding of chimeric vaccine to bovine TLR3 was validated through protein-protein docking investigations. The molecular dynamic simulation proved it to be stable in a biological system. The proposed MEV proved vital in strengthening the host immunity upon infection as the immunological simulation was projected to induce immunoglobulins, interleukins, interferons, helper T cells, and cytotoxic T cells. Our results therefore imply that the proposed vaccine will aid in inducing a strong and durable immunity in animals against BVDV infection.

To Cite This Article: Riaz R, Zahid S and Khan MS, 2024. Designing an epitope-based vaccine against bovine viral diarrhea using immuno-informatics. Pak Vet J.


ISSN 0253-8318 (Print)
ISSN 2074-7764 (Online)

SCImago Journal & Country Rank