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Estimation of In vitro Acaricidal Activities of Ethanolic and Ethyl Acetate Extracts of Nicotiana tabacum against Hyalomma species of Livestock
Eman A. Al-Nabati1, Ruoa Almahallawi2, Amal M. Alzahrani3, Nawal Al-Hoshani4*, Mariam S. Al-Ghamdi5, Sally Negm6, Attala F. El-lkott7,8, Majed A. Bajaber9, Soliman M. Soliman10, and Mohamed T. El-Saadony11

‏‏1Department of Biology, College of Science, Taibah University, Madinah, P.O. 344, Saudi Arabia; 2Department of Biology, University College of Duba, University of Tabuk, Tabuk 71491, Saudi Arabia; 3Department of Biology, Faculty of Sciences, Al Baha University, Al Baha 65779, Saudi Arabia; 4Department of Biology, College of Science, Princess Nourah bint Abdulrahman University, P.O. Box 84428, Riyadh 11671, Saudi Arabia; 5Department of Biology, College of Sciences, Umm Al-Qura University, Makkah 24381, Saudi Arabia; 6Department of Life Sciences, College of Science and Art Mahyel Aseer, King Khalid University, Abha 62529, Saudi Arabia; 7Department of Biology, College of Science, King Khalid University, P.O. Box 9004, Abha 61413, Saudi Arabia; 8Department of Zoology, Faculty of Science, Damanhour University, Damanhour 22511, Egypt; 9Department of Chemistry, Faculty of Science, King Khalid University, P.O. Box 9004, Abha 61413, Saudi Arabia; 10Department of Medicine & Infectious Diseases, Faculty of Veterinary Medicine, Cairo University, Giza 12211, Egypt; 11Department of Agricultural Microbiology, Faculty of Agriculture, Zagazig University, Zagazig 44511, Egypt
*Corresponding author:


Ticks are ectoparasites belonging to the phylum Arachnida infesting humans and animals. Besides their parasitic role, ticks also spread multiple bacterial, viral, and parasitic diseases because of their vector role. Hyalomma is a major genus of ticks containing species of zoonotic and pathogenic significance. Control of the ticks is the primary importance, but chemical acaricides are being avoided because of resistance and one health-related issue. In this study acaricidal and repellent activities of the ethanolic and ethyl acetate extracts of Tobacco (Nicotiana tabacum) against the ticks of Hyalomma genus. Each extract was serially diluted to have 1.25, 2.5, 5, and 10% concentrations. The parameters taken were adult mortality, larval mortality, egg hatchability and product effectiveness. The results suggested that the ethyl acetate and ethanolic extracts had statistically comparable (P>0.05) effects on adult mortality (tick immersion), larval mortality (larval immersion test), egg hatchability and tick repellency parameters. This research also revealed that extracts of N. tabacum were also comparable to (P>0.05) standard medicated control at the highest concentrations. Ethanolic and ethyl acetate extracts had significantly (P<0.05) different acaricidal effects from the blank control at 10% concentration. This research suggests that the N. tabacum can be used as an acaricidal and tick-repellent agent.

To Cite This Article: Al-Nabati E, Almahallawi RS, Alzahrani AM, Al-Hoshani N, Al-Ghamdi MS, Negm S, El-lkott AF, Bajaber MA, Soliman SM, El-Saadony MT, 2024. Estimation of in vitro acaricidal activities of ethanolic and ethyl acetate extracts of Nicotiana tabacum against Hyalomma species of livestock. Pak Vet J.


ISSN 0253-8318 (Print)
ISSN 2074-7764 (Online)