Raw goat milk can be a potential source to transfer antibiotic-resistant pathogens between animals and humans. Due to increased virulence and antimicrobial resistance genes acquired by Staphylococcus aureus (S. aureus), it is considered a major public health hazard and has a significant impact on the treatment and management of mastitis in dairy goats. For this purpose, Coagulase-positive S. aureus (n=103) isolates from mastitis samples in goats were evaluated to check the resistance mechanism against β-lactam antibiotics by disc diffusion assay and by PCR. The isolates were tested for the presence of coa, mecA, and blaZ genes. The results of PCR analysis revealed that out of 384 milk samples collected from goats, 103 were identified as CoPS, 37 isolates carried blaZ gene while 19 isolates were identified as methicillin-resistant Staphylococcus aureus (MRSA). The absolute frequency depicted that 13 of the isolates harbored both blaZ + mecA genes in their genetic material. The findings of the antibiogram profile of β-lactam resistant coagulase positive S. aureus was found highly resistant against the oxytetracycline, followed by gentamicin and tylosin while the maximum isolates showed sensitivity towards ciprofloxacin, levofloxacin, and moxifloxacin in both groups. The results of antibiotic susceptibility patterns would be critical for evaluating the trends and patterns of antimicrobial resistance (AMR), estimating the contribution of particular genes in drug-specific resistance, and developing control measures to decrease AMR in goats of Pakistan. The genetic analysis of the current study concluded that genetic divergence, the transfer of zoonotic MRSA, and the involvement of coa, mecA, and blaZ genes serve as important virulence factors of S. aureus leading to the causation of goat mastitis.