Fowl Typhoid (FT) is a septicemic disease caused by Salmonella Gallinaurm that affects poultry birds at any age. This study was aimed to determine the prevalence of FT and the use of bacteriophages as an effective prophylactic intervention. For this purpose, commercial layer farms located in district Layyah were visited and a total of 520 cloacal swabs and tissue samples (liver, spleen, and air sac) were collected from clinically FT-suspected birds followed by SG isolation and confirmation through conventional and molecular approach. Bacteriophages and antibiotics in diseased birds were administered and found that treated groups gained more weight, had less morbidity, and mortality than control groups. Among brown layers, 21.53% samples were found positive for Salmonella as compared to 12.69% among commercial white layers. Winter had the highest Salmonella distribution (18.46%) among white layers, followed by autumn (13.84%), spring (10.76%), and summer (7.69%). The disease was reduced by the bacteriophage supplementation, as it enhanced overall growth performance and reduced disease burden by elevating immunity levels. Phages improved development and immunological response, indicating their potential as an alternative to antibiotics for avoiding fowl typhoid in layer chickens.