Sarcocystis (S.) is a cyst-forming parasite with over 200 species in the phylum Apicomplexa. This study aimed to investigate the prevalence of S. fusiformis in cattle and examine its impact on the animals. A total of 200 cattle carcasses were examined for the presence of S. fusiformis in various tissues, including the esophagus, heart, tongue, and skeletal muscles, to assess the extent of infection. The study also focused on analyzing the associated immunological and histopathological changes in infected animals. Infected tissues, including the esophagus, hearts, and muscles, were subjected to light microscopy, transmission electron microscopy (TEM), and histopathological examination. A 10% prevalence of S. fusiformis was found among the examined cattle. Toluidine blue-stained sections revealed that the sarcocyst walls ranged from 1.8 to 6.5μm in thickness and displayed branching villar protrusions. These protrusions were observed to have homogeneous, papillomatous forms with a width of 100 nm. TEM analysis revealed filamentous tubular structures within the sarcocyst walls, some reaching lengths up to 6μm. These tubules ran parallel to the long axis of the villar projections and lacked granules. Furthermore, the expression levels of various cytokines in the infected tissues were significantly elevated, including tumor necrosis factor-alpha (TNF-α), interleukin-1β (IL-1β), and interferon-gamma (IFN-ɤ). Histopathological examination revealed tissue changes, with a small number of mononuclear inflammatory cells present in the affected organs, indicating an immune response to the parasite. This study provides valuable insights into the unique characteristics of S. fusiformis sarcocysts, helping to distinguish them from other Sarcocystis species found in both domestic and wild ruminants. Further research is recommended to explore potential control measures and better understand the broader implications of S. fusiformis infections in livestock.