PAKISTAN VETERINARY JOURNAL

Pak Vet J, 2024, 44(4) 1053-1062

In Vitro Anthelmintic Potential of Selected Essential Oils against Gastrointestinal Nematodes of Sheep

Filip Štrbac^1*, Slobodan Krnjajić¹, Nataša Simin², Dejan Orčić², Laura Rinaldi³, Vincenzo Musella⁴, Fabio Castagna⁴, Radomir Ratajac⁵, Dragica Stojanović⁶, Felwa Abdullah Thagfan⁷, Fatma Mohamed Ameen Khalil⁸ and Antonio Bosco^3*

¹Institute for Multidisciplinary Research, University of Belgrade, Kneza Višeslava 1, 11030 Belgrade, Serbia, ²Department of Chemistry, Biochemistry and Environmental Protection, Faculty of Sciences, University of Novi Sad, Trg Dositeja Obradovića 3, 21102 Novi Sad, Serbia, ³Department of Veterinary Medicine and Animal Production, University of Naples Federico II, CREMOPAR, Via Federico Delpino 1, 80137 Naples, Italy, ⁴Department of Health Sciences, University of Catanzaro Magna Græcia, Campus "S. Venuta", 88100, Catanzaro, Italy, ⁵Scientific Veterinary Institute Novi Sad, Rumenački put 20, 21113 Novi Sad, Serbia, ⁶Department of Veterinary Medicine, Faculty of Agriculture, University of Novi Sad, Trg Dositeja Obradovića 8, 21102 Novi Sad, Serbia, ⁷Department of Biology, College of Science, Princess Nourah bint Abdulrahman University, P.O. Box 84428, Riyadh 11671, Saudi Arabia, ⁸Unit of Health Specialties, Basic Sciences and Their Applications, Applied College, King Khalid University, Mohayil Asir Abha, 61421, Saudi Arabia
*Corresponding author: filip.strbac@imsi.bg.ac.rs (FŠ); antonio.bosco@unina.it (AB)

Abstract

Gastrointestinal nematodes (GINs) represent major obstacle to modern sheep farming, where the exclusive use of commercial anthelmintics is no longer a sustainable option due to the development of resistance in nematodes and the problem of drug residues in animal products and the environment. The aim of this study was to investigate the in vitro phytotherapeutic properties of seven essential oils (EOs) against GINs in sheep. Their chemical composition was determined by gas chromatography-mass spectrometry (GC-MS) analysis. The anthelmintic potential was evaluated using the egg hatch test performed at eight concentrations for each oil (50, 12.5, 3.125, 0.781, 0.195, 0.049, 0.025 and 0.0125 mg/mL). Additionally, a coproculture examination was performed to identify the GIN genera present in the tested faecal samples. The tested EOs showed a dose-dependent (R² close to 1) ovicidal activity with basil - Ocimum basilicum L. (23.3-93.3%, IC₅₀=0.08 mg/mL), spearmint - Mentha spicata L. (13.0-92.7%, IC₅₀=0.07 mg/mL) and hyssop - Hyssopus officinalis L. (42.7-91.3%, IC₅₀=0.19 mg/mL) being the most effective. The high activity of these oils could be attributed to the high content of the monoterpene alcohol linalool (O. basilicum) and the terpenoid ketones carvone (M. spicata) and pinocamphone (H. officinalis). Four genera of sheep GINs were identified on coproculture examination, i.e. Haemonchus 43%, Trichostrongylus 35%, Teladorsagia 17% and Chabertia 5%. The obtained results suggest that EOs of basil, spearmint and hyssop could represent a valuable alternative in the control of GINs in sheep, which could help to reduce the use of chemical drugs. Future field trials should be performed to confirm these findings.

To Cite This Article: Štrbac F, Krnjajić S, Simin N, Orčić D, Rinaldi L, Musella V, Castagna F, Ratajac R, Stojanović D, Thagfan FA, Khalil FMA and Bosco A, 2024. In vitro anthelmintic potential of selected essential oils against gastrointestinal nematodes of sheep. Pak Vet J, 44(4): 1053-1062. http://dx.doi.org/10.29261/pakvetj/2024.295

Abstract

ISSN 0253-8318 (Print)ISSN 2074-7764 (Online)

ISSN 0253-8318 (Print)
ISSN 2074-7764 (Online)