Therapeutic use of natural products is currently considered as a global trend, highlighting the need for further research to develop more effective natural alternatives. The Frankincense aquas extract (FAE) contained high levels of boswellic acids (43.2%) and volatile compounds, i.e., thunbergol (37.2%); therefore, FAE scavenged 92% of 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radicals, inhibited 84 and 86% of the viability of HePG-2 and MCF-7 cancer cell lines, the FAE (20-35µg/mL) inhibited the multidrug-resistant bacteria (MDRB). In this study, the Frankincense (5% w/v) aquas extract (FAE) was evaluated for its antioxidant, antibacterial, anticancer, and anti-inflammatory potential in glycerol-induced acute kidney injury (AKI) in 24 adult male Wistar rats. The experimental rats were randomly divided into four groups: (1) control, (2) AKI glycerol (50%, 10 mL/kg i.m.), (3) oral FAE (5% w/v), and (4) AKI glycerol+FAE two times daily through oral gavage for four weeks. Administrating FAE in AKI rats led to a significant improvement in renal function parameters (uric acid, urea, Neutrophil Gelatinase-Associated Lipocalin-NGAL), total proteins (TP), and cystatin C, with no effect on creatinine levels compared to the control group. Furthermore, it enhanced (p<0.05) GSH, SOD, and NO levels and decreased MDA levels, indicating that FAE improved the oxidative status of rats. Compared to the normal, FAE treatment also downregulated inflammatory markers, i.e., tumor necrosis factor-alpha (TNF-α) and Interleukin 6 (IL-6) in AKI rats. The kidney sections showed typical structure in FAE treated healthy rats as in control, and the AKI-FAE group showed a significant reduction in focal inflammatory cells. It was concluded that oral administration of FAE (5% w/v) for 28 days restored renal function in the AKI rat model by reducing inflammation and oxidative stress.