The role of FOXM1 in ferroptosis remains largely unknown. We aimed to explore the role of forkhead box M1 (FOXM1) suppression in promoting the ferroptosis of gastric cancer cells.Groups were set for SGC-7901 cells, including si-FOXM1 group, si-FOXM1+Fer-1 group, si-NC group, si-SUV39H1 group, si-SUV39H1+Fer-1 group, si-FOXM1+pcDNA3.1+SUV39H1 group, si-FOXM1+pcDNA3.1+SUV39H1+Fer-1 group, and control group. CCK-8 method was employed to determine the proliferation of cells. ELISA was conducted to measure the iron content in cells. In comparison with the control group, the cell survival rate, hydrogen sulfide (H₂S) yield and protein expressions of SUV39H1, cystathionine γ-lyase (CSE), glutathione peroxidase 4 (GPX4) and solute carrier family 7 member 11 (SLC7A11) declined significantly in the si-FOXM1 group, while the relative fluorescence intensity of reactive oxygen species (ROS) and the relative iron content increased (P<0.05). Si-FOXM1+pcDNA3.1+SUV39H1 and si-FOXM1+pcDNA3.1+SUV39H1+Fer-1 groups, especially the latter group, had significantly higher cell survival rate, H₂S yield and protein expressions of SUV39H1, CSE, GPX4 and SLC7A11, and significantly lower relative fluorescence intensity of ROS and relative iron content than those of the si-FOXM1 group (P<0.05). FOXM1 suppression is capable of acting on gastric cancer cells through proliferation inhibition combined with ferroptosis promotion.

To Cite This Article: Li Z, Hou W, Wang J, Han X, Zhang L, Tariq M and Wang X, 2024. FOXM1 inhibits SUV39H1 to regulate the CSE/H2S pathway in promoting ferroptosis of gastric cancer cells. Pak Vet J. http://dx.doi.org/10.29261/pakvetj/2025.001