PAKISTAN
VETERINARY
JOURNAL
     
 
previous page   Pak Vet J, xxxx, xx(x) xxxx   next page
 
In-vitro, in-vivo and whole genome-based probe into probiotic potential of Weissella viridescens PC-45 against Salmonella Gallinarum
 
Adnan Mehmood1, M. Nawaz*1, Masood Rabbani1 and M. Hassan Mushtaq2
 

1Institute of Microbiology, University of Veterinary and Animal Sciences, Lahore, 54000, Pakistan. 2Department of Epidemiology and Public Health, University of Veterinary and Animal Sciences, Lahore, 54000, Pakistan
*Corresponding author: muhammad.nawaz@uvas.edu.pk

Abstract   

This study was aimed to isolate and screen Weissella spp. with anti-Salmonella Gallinarum (S. Gallinarum) activity from the poultry gut and to analyze their probiotic properties from phenotypic and genomic characterization aspects in-vitro and in-vivo in broiler. Six isolates were confirmed as Wiessella by 16S rDNA sequencing. These isolates showed variable in-vitro activity (10.00±0.57~17.00±1.00 mm) against S. Gallinarum and were further evaluated for probiotic traits. In-vitro, results revealed that Weissella viridescens (W. viridescens) PC-45 can tolerate acidic conditions (pH 3 and 4), resist bile salts (>0.5 OD at 600nm), auto-aggregate (58.98%±0.85), co-aggregate with S. Gallinarum (47.23±0.94%) and have no acquired antibiotic resistance. The strain also inhibited (>4 log10) S. Gallinarum in co-culture assays. Genomic analysis of W. viridescens PC-45 revealed a genome size of 1593258 bp with a 41% GC contents. Genes related to probiotic properties, including resistance to acidic pH, bile salts, oxidative stress, synthesis of exopolysaccharide, host-microbe interaction, and resistance to stress conditions, were identified by the Kyoto Encyclopedia of Genes and Genomes (KEGG). Moreover, it also contained various CRISPR-associated genes and no genes for acquired antibiotic resistance. In-vivo results revealed that W. viridescens PC-45 reduced (>2 log10) S. Gallinarum counts in broilers. It is concluded that this strain may be used to further develop anti-S. Gallinarum probiotic products to control fowl typhoid.

To Cite This Article: Mehmood A, Nawaz M, Rabbani M and Mushtaq MH, 2024. In-vitro, in-vivo and whole genome-based probe into probiotic potential of Weissella viridescens PC-45 against Salmonella Gallinarum. Pak Vet J. http://dx.doi.org/10.29261/pakvetj/2024.306

 
 
   
 

ISSN 0253-8318 (Print)
ISSN 2074-7764 (Online)



scopus
 
DOI
 
DOAJ SEAL
  
SCImago Journal & Country Rank