In-vitro,
in-vivo and whole
genome-based probe into probiotic potential of
Weissella viridescens
PC-45 against Salmonella
Gallinarum
Adnan Mehmood1, M. Nawaz*1, Masood Rabbani1
and M. Hassan Mushtaq2
1Institute
of Microbiology, University of Veterinary and Animal Sciences,
Lahore, 54000, Pakistan. 2Department of Epidemiology and
Public Health, University of Veterinary and Animal Sciences, Lahore,
54000, Pakistan
*Corresponding author:
muhammad.nawaz@uvas.edu.pk
Abstract
This study was aimed to isolate and screen
Weissella spp. with anti-Salmonella
Gallinarum (S. Gallinarum)
activity from the poultry gut and to analyze their probiotic properties from
phenotypic and genomic characterization aspects
in-vitro and
in-vivo in broiler. Six isolates were
confirmed as Wiessella by 16S rDNA sequencing. These isolates showed
variable in-vitro activity
(10.00±0.57~17.00±1.00 mm) against S.
Gallinarum and were further evaluated for probiotic traits.
In-vitro, results revealed that
Weissella viridescens (W.
viridescens) PC-45 can tolerate acidic conditions (pH 3 and 4), resist bile
salts (>0.5 OD at 600nm), auto-aggregate (58.98%±0.85), co-aggregate with
S. Gallinarum (47.23±0.94%) and have
no acquired antibiotic resistance. The strainalsoinhibited (>4 log10)
S. Gallinarum in co-culture assays.
Genomic analysis of W. viridescens
PC-45 revealed a genome size of 1593258 bp with a 41% GC contents. Genes related
to probiotic properties, including resistance to acidic pH, bile salts,
oxidative stress, synthesis of exopolysaccharide, host-microbe interaction, and
resistance to stress conditions, were identified by the Kyoto Encyclopedia of
Genes and Genomes (KEGG). Moreover, it also contained various CRISPR-associated
genes and no genes for acquired antibiotic resistance.
In-vivo results revealed that
W. viridescens PC-45 reduced (>2
log10) S. Gallinarum counts in
broilers. It is concluded that this strain may be used to further develop anti-S.
Gallinarum probiotic products to control fowl typhoid.
To Cite This Article:
MehmoodA, NawazM, RabbaniM and MushtaqMH, 2024. In-vitro, in-vivo
and whole genome-based probe into probiotic potential of
Weissella viridescens PC-45 against
Salmonella Gallinarum. Pak Vet J.
http://dx.doi.org/10.29261/pakvetj/2024.306