Isolation, genome phylogenetic analysis and
in vitro rescue of a
newly emerging porcine circovirus type 2
Weijuan Zhu and Xiaofeng Ren*
College of Veterinary Medicine, Northeast Agricultural University,
59 Mucai Street, Xiangfang District, 150030 Harbin, China
*Corresponding author: rxfemail@yahoo.com.cn; renxf@neau.edu.cn
Abstract
Porcine circovirus type 2 (PCV2) is the major causative agent of postweaning
multisystemic wasting syndrome (PMWS). Infection by PCV2 may cause heavy losses
in pig industry. In this study, we report the isolation of a newly emerging PCV2
from northeastern China. The complete genome of the PCV2 isolate named PCV2-LJR
contains 1766 nucleotides and was compared with reference sequences published in
GenBank followed by topology analysis of the resulting phylogenetic tree. The
data indicated that the prevalent PCV2 isolates in the northeastern China had
close relationship, although various genotypes of PCV2 existed. In addition, by
gene recombination and transfection techniques, the PCV2 infectious clone was
achieved and was able to rescue virus in
vitro determined by indirect immunofluorescence assay and PCR. The obtained
biological materials may be used for biological characterization of PCV2.