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Isolation, genome phylogenetic analysis and in vitro rescue of a newly emerging porcine circovirus type 2
 
Weijuan Zhu and Xiaofeng Ren*
College of Veterinary Medicine, Northeast Agricultural University, 59 Mucai Street, Xiangfang District, 150030 Harbin, China *Corresponding author: rxfemail@yahoo.com.cn; renxf@neau.edu.cn

Abstract   

Porcine circovirus type 2 (PCV2) is the major causative agent of postweaning multisystemic wasting syndrome (PMWS). Infection by PCV2 may cause heavy losses in pig industry. In this study, we report the isolation of a newly emerging PCV2 from northeastern China. The complete genome of the PCV2 isolate named PCV2-LJR contains 1766 nucleotides and was compared with reference sequences published in GenBank followed by topology analysis of the resulting phylogenetic tree. The data indicated that the prevalent PCV2 isolates in the northeastern China had close relationship, although various genotypes of PCV2 existed. In addition, by gene recombination and transfection techniques, the PCV2 infectious clone was achieved and was able to rescue virus in vitro determined by indirect immunofluorescence assay and PCR. The obtained biological materials may be used for biological characterization of PCV2.

Key words: Genome, Porcine circovirus type 2, Virus isolation, Virus rescue

 
   

ISSN 0253-8318 (Print)
ISSN 2074-7764 (Online)



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