Histochemical Localization of the Cholinergic and Nitrergic Neurons
in the Chicken Ileum
Ping Yang, Jameel Ahmed Gandahi§, Xunguang Bian,
Li Wu,
Shamsuddin Bughio1,
Linli Zhang and Qiusheng Chen*
Laboratory of Animal Cell Biology and Embryology,
College of Veterinary
Medicine, Nanjing Agricultural University, Nanjing, PR China; 1Department of Veterinary Pharmacology, Sindh Agriculture
University, Tandojam, Pakistan; §Also
associated with:Department of Anatomy & Histology, Sindh
Agriculture University, Tandojam, Pakistan * Corresponding author:
ypnjau@yahoo.com.cn; qschen1@yahoo.com
Abstract
Histochemical loacalization and analysis of the cholinergic and nitrergic
neurons in the chicken ileum were investigated by staining with acetylcholine
esterases (AChE) and nicotinamide adenine dinucleotide phosphate diaphorase
(NADPH-d), respectively. AChE and NADPH-d activity was demonstrated in neuronal
cell bodies and nerve fibres in the chicken ileum. The positive neurons showed
irregular or polygonal shape and were mainly present sporadic or clumped in the
myenteric and submucosal plexus. The positive nerve fibres frequently surrounded
the ileac blood vessels. They were abundantly present in myenteric and
submucosal plexus of the ileum forming a network. Some positive nerve fibres
traversed the submucosa into the lamina propria mucosae. Fine nerve fibres were
found to penetrate into intestinal villi underneath the epithelium. Extensive
networks of more intensely staining AChE positive nerve fibers were present in
the mucosa as compared to that of NADPH-d positive fibers. Ganglia density of
submucosal plexus was markly biger than that of myenteric plexus, whereas
neurons per ganglion and the number of neurons per mm2 and the size
of neurons of submucosal plexus were shorter than that of myenteric plexus. In
addition, the number of AChE positive neurons and nerve fibres was more than
that of NADPH-d positive neurons and nerve fibres. We concluded that the chicken
ileum is characterized by abundance of nerve structures which may play a
significant functional role in ileum of the chicken.