Effects of Different Levels of Pigeon Egg Yolk
in Extenders on the
Post-Thaw Semen Quality of Sahiwal Bulls
Hafez Jamil-ur-Rahman, Nazir Ahmad*,
Najib-ur-Rahman, Salman Waheed, Maqbool Ahmad, Muhammad Younis1
and Tanveer Ahmad2
Department of Theriogenology, University of
Agriculture, Faisalabad, Pakistan; 1Semen Production
Unit, Qadirabad, Sahiwal, Pakistan; 2Department of
Clinical Medicine & Surgery, University of Agriculture, Faisalabad,
Pakistan *Corresponding author: profnazir53@hotmail.com
Abstract
In this study, effects of
replacing chicken egg yolk (CEY) with pigeon egg yolk (PEY)
in extenders on post-thaw semen
quality in Sahiwal bulls were investigated. Attempts were also made to see if
post thaw semen quality was affected by reducing PEY level in the extender.
Twenty four semen samples were diluted with five Tris-based extenders. Extender
A contained 20% CEY and was used as control, while extenders B, C, D and E
contained 5, 10, 15 and 20% PEY, respectively. After freezing and storage for 24
hrs in liquid nitrogen, these samples were evaluated for post-thaw semen quality
parameters. The difference in post extension sperm motility between
extenders A (20% CEY) and E (20% PEY) was non significant. Post extension sperm
motility decreased as the level of PEY in the extender was decreased. A similar
trend was recorded for post thaw sperm motility, livability, absolute index of
livability and sperm with intact plasma membrane. The percentages
of spermatozoa with abnormal head, or tail were lower (P<0.01) in control
extender A and extender E compared to extenders B, C and D. However, for
abnormal mid-piece, extenders A and E showed lower values than extender C only.
It was concluded that replacing CEY with PEY in same concentration (20%) did not
improve post thaw semen quality. Moreover, reducing the concentration of PEY in semen
extender from 20 to 5% had adverse effects on post-thaw quality of Sahiwal bull
semen.