PAKISTAN VETERINARY JOURNAL

Pak Vet J, 2012, 32(3): 447-450

Antibody against Porcine Parvovirus VP2 Protein Differentiated This Virus from Other Viruses

Weijuan Zhu^§, Yudong Ren^§¹, Guangxing Li, Dingding Su², Qing Yang³ and Xiaofeng Ren*

College of Veterinary Medicine; ¹College of Engineering, Northeast Agricultural University, 59 Mucai Street, Xiangfang District, Harbin 150030, China; ²Hunan Provincial Key Laboratory for Germplasm Innovation and Utilization of Group; ³College of Veterinary Medicine, Hunan Agricultural University, 1 Nongda Road, Furong Disctrict, Changsha 410128, China
^§These authors equally contributed to this study
* Corresponding author: E-mails: rxfemail@yahoo.com.cn

Abstract

Viral protein 2 (VP2) of porcine parvovirus (PPV) is the major viral structural protein and responsible for eliciting neutralizing antibodies in immunized animals. In this study, the gene encoding VP2 of PPV was amplified by PCR. The VP2 gene was then cloned into the prokaryotic expression vector, pET-32a followed by expression in Escherichia coli Rosetta. The VP2 protein expression was analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Rabbit polyclonal antiserum was generated using the recombinant VP2 protein. The optimal titer of the anti-VP2 antibody was determined by ELISA. The anti-VP2 antibody was able to distinguish PPV from other viruses in ELISA.

Key words: Antibody, ELISA, Porcine parvovirus, VP2

Abstract

ISSN 0253-8318 (Print)ISSN 2074-7764 (Online)

ISSN 0253-8318 (Print)
ISSN 2074-7764 (Online)