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Evaluation of the Difference of L-selectin, Tumor Necrosis Factor-a and Sialic Acid Concentration in Dairy Cows with Subclinical Ketosis and without Subclinical Ketosis
Z. G. Zhang1,§, J. D. Xue§2,§,  R. F. Gao3,§, J.Y. Liu1,§, J. G. Wang3, C. Y. Yao2, Y. Liu2, X. W. Li3, X. B. Li3, G. W. Liu3 and Z. Wang3,*
1College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030,China; 2College of Animal Science and Technology, Inner Mongolia University for Nationalities, Tongliao 028000, China; 3College of Animal Science and Veterinary Medicine, Jilin University, Changchun 130062, China
*Corresponding author:


Ketosis is a major disease related with negative energy balance and immune suppression in dairy cows. The objective of this study was to examine the differences in β-hydroxybutyrate (BHBA), L-selectin, glucose, tumor necrosis factor-α (TNF-α), non-esterified fatty acids (NEFA), and sialic acid (SA) concentrations in serum in healthy dairy cows and those with subclinical ketosis during the early lactation period. The blood from 20 healthy cows and 20 subclinically affected cows were sampled. All the cows were within the first 2 months of lactation. Serum concentrations of the various aforementioned factors were measured using a number of different methods. The results demonstrated that in cows affected by subclinical ketosis, NEFA concentrations were significantly higher, and glucose and L-selectin concentrations were significantly lower than healthy cows. There was no significant difference in serum SA and TNF-α of dairy cows with subclinical ketosis compared to the control cows. The decrease in concentration of serum L-selectin may be related to immune suppression.

Key words: Immune suppression, L-selectin, Subclinical ketosis, Sialic acid, TNF-a


ISSN 0253-8318 (Print)
ISSN 2074-7764 (Online)