Determination of Ovsynch Efficiency for Oestrus
Synchronization by Plasma LH and P4 Levels in Nili Ravi Buffalo during Peak and Low Breeding Seasons
S. Jabeen, M. Anwar1*,
S. M. H. Andrabi1, A. Mehmood1,
S. Murtaza2and M. Shahab
Laboratory of Reproductive Neuroendocrinology,
Faculty of Biological Sciences, Quaid-i-Azam University, Islamabad;
1Animal Reproduction Program, Animal Sciences
Institute, National Agricultural Research Centre, Park Road,
Islamabad-45500; 2Nuclear Medicine, Oncology and
Radiotherapy Institute, Hanna Road, G-8/3, Islamabad, Pakistan
*Corresponding author: manwar_94@yahoo.com
Abstract
Oestrus synchronization may be used to overcome
poor oestrus expression and seasonality of breeding in buffalo (Bubalus
bubalis). The present work was conducted to compare the efficiency of an
oestrus synchronization protocol “ovsynch” in Nili Ravi buffalo during peak
(n=8) and low breeding seasons (n=11) by determining luteinizing hormone (LH)
peak and progesterone (P4) rise in blood plasma using ELISA. Buffaloes were
administered gonadotropin releasing hormone (GnRH) analogue (50 µg lecirelin;
day 0) followed by prostaglandinF2α (PGF2α) analogue (150 µg cloprostenol; day
7) and again GnRH analogue at 36 hours after PGF2α. Blood sampling for LH was
started 12 h after PGF2α injection and done at 3 h interval up to 108 h. An
animal was considered to have responded to ovsynch protocol if it showed LH peak
within 48 h after PGF2α injection (and
within 3-6 h after second GnRH injection)” and showed a P4 concentration
of >2.0 ng/ml on
day 18 after the 1st GnRH injection.87.5% animals showed positive response
to ovsynch protocol in term of oestrus and ovulation synchronization during the
peak season as assessed by LH peak and P4 level while only 36.36 % animals
responded to synchronization treatment during low breeding season (P<0.05).
The LH peak in responsive animals was 11.48±1.98 and 10.38±1.77 ng/ml in
peak and low breeding season respectively. It is concluded that
Ovsynch protocol in buffaloes resulted in a significantly better
response during peak breeding season as compared to that in low breeding season.
Key words: Buffalo,
LH,
Oestrus synchronization, Progesterone, Season