Evaluation of the Genotoxicity of
Bangpungtongsung-San, a Traditional Herbal Prescription
In-Sik Shin§, Chang-Seob Seo§,
Mee-Young Lee, Hye-Kyung Ha, Ji-Young Kim1, Jung-Im Huh1
and Hyeun-Kyoo Shin*
Herbal Medicine Formulation Research Group, Korea Institute of Oriental
Medicine, 483 Expo-ro, Yusung-gu, Daejeon 305-811; 1Division
of Non-clinical Studies, Korea Institute of Toxicology, P.O. BOX
123, 100 Jangdong, Yusung-gu, Daejeon 305-343, Republic of Korea *Corresponding author: hkshin@kiom.re.kr
Abstract
Bangpungtongsung-san (BPS) is a traditional
Korean herbal formula used as an anti-inflammatory, antipyretic, antiobesity,
and choleretic agent, which consists of 18 herbs. As part of a safety evaluation
of BPS, the present study evaluated the potential genotoxicity of an aqueous BPS
extract using a standard battery of tests, including, the Ames test, chromosomal
aberration test, and mouse micronucleus test. The BPS extract was not found to
be genotoxic under the conditions of the Ames test. In micronucleus test, oral
administration of BPS at doses up to 2,000 mg/kg did not increases the incidence of
micronucleated polychromatic erythrocyte. The chromosomal aberration test showed
that the BPS extract induced an increase in the number of structural and
numerical chromosomal aberrations in the group treated with BPS at high dose
levels (2,500 and 4,000 μg/mL) for 6 h, in the presence of the
metabolic activation system (S-9 mix), compared with the vehicle control. In
conclusion, these results indicate that BPS extract may act as a genotoxic
agent.