Relationship between Disease Activity and Circulating Level of
Collagen II C-Telopeptide Fragments in Papain Induced Osteoarthritis
Rat Model
Humaira Majeed Khan1, Muhammad Ashraf1,
Abu Saeed Hashmi2, Mansur-ud-Din Ahmad2* and
Aftab Ahmad Anjum2
1Lahore
College for Women University, Lahore; 2University of
Veterinary and Animal Sciences, Lahore-54000, Pakistan.
*Corresponding author:
mansuruddin@uvas.edu.pk;
mansuruddin@gmail.com
Abstract
Osteoarthritis (OA) is a progressive degeneration
of articular cartilage leading to failure in functional mobility of joints. It
is characterized by morphological, biochemical and molecular changes in
histology of cartilage. Different biological markers are used as indicators to
precisely predict the stage of cartilage destruction of joints in OA patients
and to evaluate the therapeutic efficacy of drugs used for OA. The present
research was chalked out to establish relationship between disease activity and
serum level of C-terminal telopeptide of type II collagen (CTX-II) in
experimentally induced OA rat model. Out of 30 male Wistar rats, 25 were used to
induce OA by injecting papain (10mg/0.5mL of 0.05M sodium acetate) in right knee
joints whereas five (control) were injected with sterile normal saline solution
on day 0. Blood samples (5mL each) were collected on weekly basis up to 28th
days of post papain injection. Sera were separated and subjected to perform
ELISA for estimating CTX-II fragments as cartilage biomarker (CartiLaps ® ELISA
kit) in experimental groups. Maximum level of CTX–II (pg/mL) (40.44±3.07) was
observed in sera samples of day 14 post papain injection followed by days 21
(40.22±2.01), 28 (36.82±3.81), 7 (34.48±4.17), 1 (15.08±4.22) and day 0
(2.55±0.10). The early changes in serum CTX-II from day 0 to 14 showed
significant association with cartilage damage. Later on, no significant
difference was observed in CTX-II level on day 14, 21 and 28 post papain
injection. It is concluded that elevation in serum CTX-II level was concomitant
with the onset of disease and degradation of cartilage. Moreover, CTX-II is a
sensitive diagnostic biomarker to monitor joint disorder severity in papain
induced OA rat experimental model on different days. These findings may be used
as base line for early diagnosis of disease and initiation of therapy for
successful outcome.