Testing of Some Canine Blood Types in Transfusion Compatibility
Assessment
L. Ognean
Department of
Department of
Physiology, Faculty of Veterinary Medicine, University of
Agricultural Science and Veterinary Medicine, 3-5 Mănăştur street,
400372 Cluj-Napoca, România *Corresponding author:
lognean@yahoo.com
Abstract
Blood types were determined using SHIGETA (n=136)
and DEA1.1 (n=25) kits, in two
groups of dogs, consisting of patients that underwent blood transfusions and
healthy donors. The tests were conducted in accordance with the procedures
established by the manufacturers, using specific monoclonal antibodies kits,
heparinized blood for the tube agglutination (TUBE) and slide (SLIDE) methods,
and EDTA treated blood for the CARD and chromatographic (CHROM) methods. The
clear expression of tube agglutination reaction in the SHIGETA kit provided a
good detection of antigens. Positive reactions with anti-DEA1.1 were clear and
evident with the CHROM test. SHIGETA tests revealed a predominance 1.1B (47.05%)
of blood type, common in Rotweilers (81.81%) and Romanian Shepherds (73.68%) and
group 1(-)B (24.26%), frequently found in German Shepherds (54.16%), these also
representing an important source of compatible blood. DEA1.1 type test, revealed
a high frequency of positive dogs (75%), associated with lower number of
potential donors. Extrapolation of SHIGETA groups into the DEA system, confirmed
the 1(-)B positive dogs as DEA 1.1 negative, and their prevalence in German
Shepherds also confirmed their known tendency to be “ideal donors”.
The CHROME test showed a good efficiency in
auto agglutination control and detecting DEA1.1 positive dogs, including
patients with severe forms of anemia.