PAKISTAN
VETERINARY
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previous page   Pak Vet J, 2014, 34(1): 100-103   next page
 
Investigation on the Genotoxicity of Mercuric Chloride to Freshwater Clarias gariepinus
 
Shahid Mahboob*, Hmoud Fares Alkkahem Al-Balwai, Fahad Al-Misned and Zubair Ahmad
Department of Zoology, College of Science, King Saud University, P.O. Box 2455, Riyadh-11451, Saudi Arabia
*Corresponding author: shahidmahboob60@hotmail.com

Abstract   

The cytogenetic effect of heavy metal was studied in Clarias gariepinus using the micronucleus test, chromosomal aberrations and sister chromatid exchange. The fish were kept separately and treated with four different concentrations of mercuric chloride for a period of 7 days. For the micronucleus test blood samples were obtained from the caudal vein. The mean micronuclei frequencies were recorded as 0.20, 0.25, 0.50, 0.65 and 0.79 in controls and 1.0, 2.0, 4.0 and 6.0 ppm in groups treated with mercuric chloride, respectively. Kidney tissues were used for in vivo chromosome preparation. The mean frequencies of cells with chromosomal aberrations were 0.21, 0.32, 0.49, 0.70 and 0.97 in the control and 1, 3, 5 and 7 ppm in groups treated with mercuric chloride, respectively. Similarly, the mean frequencies of sister chromatid exchange were recorded as 0.10, 0.28, 0.47, 0.58 and 0.64 in controls and 1, 3, 5 and 7 ppm in groups treated with mercuric chloride, respectively. The findings of present experiment suggest that HgCl2 caused genotoxic effects in fish.

Key words: Chromosomal aberration, Clarias gariepinus, Cytogenetics, Genotoxicity, Heavy metal, Mercuric chloride, Micronucleus test, Sister chromatid exchange

 
   

ISSN 0253-8318 (Print)
ISSN 2074-7764 (Online)



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