Effect of Fibrolytic Enzymes Produced from an Improved Mutant of
Chaetomium thermophile DG-76 on the Performance of Beetal-Dwarf
Crossbred Goat
HN Hussain*, SA Khanum, M Hussain, A Shakur and F Latif1
Nuclear Institute for Agriculture and Biology (NIAB); 1National
Institute for Biotechnology and Genetic Engineering (NIBGE), Jhang
Road, Faisalabad, Pakistan *Corresponding author:hnoubahar@yahoo.com
Abstract
This study was conducted to assess the
performance and economical benefits of use of enzyme supplementation in an
intensive feeding system in Beetal x Dwarf (crossbred) male goats. The
fibrolytic enzyme produced from Chaetomium thermophile (DG-76), contained
5.8±0.1 U/ml, 8.3±0.2 U/ml, 6.2±0.2 U/ml, 170±2.2
U/ml and 2.2±0.02 mg/ml of filter paper (FP)-ase, CMC-ase, β-glucosidase,
xylanase and extra cellular protein, respectively. The enzyme was mixed @ 1% v/w
in total mixed ration (TMR) containing 15.14% CP, 20.75% ADF and 35.24% NDF
which was offered for 60-d to two similar groups of 10 in each male goats
weighing Control 33.42±1.1 kg and fibrolytic enzyme supplemented (FES) group
34.02±1.13 kg. There was a significant improvement (P=0.0001) in weight gain in
enzyme supplemented than control group (5.01 vs 4.1 kg). Average daily live
weight gain (ADLWG) was found to be 83.49 g in FES compared with 68.33 g in
control and was statistically significant (P=0.0001). There was a
non-significant (P=0.2875) decrease of 3.75% in feed intake in control group
compared with FES, however, the feed conversion ratio (FCR) varied significantly
(P=0.0008) and was 15.08% lower in FES than the control. Overall the enzyme
supplementation resulted in 31.25% increase in net profit compared with the
control group.
Key words: Cellulase,
Fibrolytic enzymes,
Goat,
Weight gain, Xylanase