In
vivo and
In vitro Studies on the
Efficacy of Anthelmintics against
Haemonchus contortus in
Goats
Nasreen Akhter*, AG Arijo, MS Phulan and Zafar
Iqbal1
Department of Parasitology, Sindh Agriculture
University, Tandojam; 1Department of Parasitology,
University of Agriculture, Faisalabad, Pakistan.
*Corresponding author: nasreenakhter58@gmail.com
Abstract
A study was conducted to assess the efficacy of
Valbazen, Levamisole and Dectomax
against Haemonchus contortus,using fecal egg count reduction
test (FECRT) and egg hatch assay (EHA). Thirty two goats with naturally acquired
H.
contortus infection were divided into four groups of 8 goats each.
Group A was administered Valbazen (@1ml/20 kg BW) while B & C groups were
treated with Levamisole and Dectomax @
5ml/15kg (oral) & 1ml/33kg BW (sub cut), respectively. The
D group served as untreated control. Faecal samples were collected on day 0
before treatment and on day 3,7,10 and 14 post treatment. The overall mean
percent of fecal egg counts (FECs) on day 10th and 14th
post treatment showed significant decrease (P<0.05) in fecal egg count. About
91.8% efficacy against H. contortus
was recorded in goats with Dectomax followed by Valbazen
(88.6%) and Levamisole (83.4%) on day 14th post treatment suggesting
Dectomax as the most effective of the three anthelmintics. The Benzimidazole
(pure) was evaluated for its ability to inhibit egg hatching in
H. contortus and lethal dose (LD50)
was calculated from linear regression. The eggs (200/1ml) were incubated with
different concentrations (3.0, 1.5, 0.75, 0.375, 0.1875, 0.0937, 0.0468, 0.0234,
0.0117 and 0.0058 µg/ml) at 22ºC for 48 h. The inhibition of egg hatching at
different concentrations ranged from 3.5 to 81.2% and LD50 was 0.1202
µg/ml which is higher than the recommended 0.1 µg/ml. The present data suggest
that all the three anthelmintics used in present study showed reduced efficacy
against H. contortus in goats than WAAVP level of 95%. Further detailed
studies are necessary to clarify the current status of efficacy of three
anthelmintics used in the present study.