Expression of Clusterin Isoforms in LPS Treated
Porcine Aortic Endothelial Cells
Augusta Zannoni*, Andrea
Zaniboni, Chiara
Bernardini, Eraldo Seren, Maria Laura Bacci and Monica Forni
Department of Veterinary Medical Sciences –
DIMEVET- University of Bologna, Bologna, Italy
Abstract
Clusterin (CLU) is a multifunctional protein
whose expression has been related to many physiological and pathological
processes. The most abundant gene product is the secreted form of CLU (sCLU)
described as a prosurvival and cytoprotective protein. A nuclear CLU (nCLU)
isoform involved in death and inhibition of cell growth has also been reported.
The aims of the present work were to verify the differential presence of sCLU
and nCLU in a primary culture of porcine aortic endothelial cells (pAEC) and to
evaluate whether lipopolysaccharide (LPS) can influence their expression.
LPS-treated pAEC expressed both mRNA isoforms but only sCLU protein was
detected. LPS exposure induced transient mRNA and protein increases of sCLU at 1
h of treatment. In conclusion our data showed that in LPS-treated pAEC sCLU is
the most abundant form of clusterin and nCLU can be considered irrelevant and is
rarely translated.
Key words:
Clusterin,
LPS,
pAEC, Small heat shock proteins