Nitrate
Provoked Kidney Toxicity and DNA Impairment in Adult Rats:
Alleviation byHyparrhenia hirta
Hanen Bouaziz1, Moez Rafrafi§2,
Ghada Ben Salah§3, Rim Marrekchi4, Ons
Boudawara5, Hassen Kammoun2, Kamel Jamoussi4,
Tahia Boudawara5 and Najiba Zeghal1*
1Animal
Physiology Laboratory; 2Laboratory of Chemistry, Sfax
Faculty of Sciences, University of Sfax, BP1171, 3000 Sfax, Tunisia;
3Laboratory of Human Molecular Genetics, Sfax Faculty of
Medicine, 3029 Sfax, Tunisia; 4Laboratory of
Biochemistry; 5Histopathology Laboratory, University of
Sfax, CHU Habib Bourguiba, 3029 Sfax, Tunisia;
*Corresponding author:
najiba.zeghal@tunet.tn;§These authors contributed equally
to this work
Abstract
Our research explored the effects of
Hyparrhenia hirta (H. hirta) against kidney impairment caused by
nitrate. Experimentation was performed on rats separated in three groups. Sodium
nitrate (NaNO3) was supplied everyday by gavage to treated groups
(400 mg/kg BW) either alone, or co-administered with methanolic extract of H.
hirta (200 mg/kg bw).
Nephrotoxicity caused by sodium nitrate was objectified by an increase in plasma
and a reduction in urine concentrations of urea and creatinine, while uric acid
level decreased in plasma and increased in urine when compared to controls. Our
results showed also, a significant decrease in clearance of creatinine in NaNO3-treated
group. In addition, sodium nitrate produced oxidative stress in the kidney
characterized by enhanced lipid peroxidation, reduced total glutathione level
and catalase, superoxide dismutase and glutathione peroxidase activities. Sodium
nitrate provoked fragmentation of DNA as reflected by smear and no formation of
ladder. Renal damages were characterized histologically after nitrate treatment
by degeneration of renal tubule cells and mononuclear cells infiltration.
Co-administration of H. hirta improved biomarkers of kidney toxicity,
malondialdehyde level, activities of antioxidant enzymes and histological
damages. We concluded that H. hirta had a significant role in animal
protection from nitrate-induced kidney dysfunction.