Responses of Chicken Sertoli Cells and
Fibroblasts after Transfection with Plasmids pEGFP-N3-HNP-1
Ahmed Khalid1,2, Yu Na1,
Zhang Jinyou,1 Nagam Khudhair3,4, Zheng peng1,
Zhao xunwu1 and Zhang Guixue1,*
1College
of Animal Science and Technology, Northeast Agricultural University,
Harbin 150030, China; 2Department of Animal Production,
College of Agriculture, University of Tikrit, Iraq; 3Key
laboratory of Dairy Science of Education Ministry, Northeast
Agricultural University, Harbin 150030, China. 4Biology
Department, Education College for Women, Al-Anbar University, Ramadi,
31001, Iraq *Corresponding author:gxzhang@neau.edu.cn
Abstract
Chicken Sertoli cells (SCs) and fibroblast cells
(FCs) were transfected with two different plasmid vectors to study their
comparative responses to transfection and to heterogenous protein appeared in vitro cultures of both cell lines. Sertoli cells and FCs
(control) were transfected with plasmids pEGFP-N3-HNP-1 and pEGFP-N3 and
efficacy was recorded. Subcellular localization of both proteins was observed.
IL-1β, IL-1RN, Fas, FasLG (FasL) and Caspase-3 expressions were examined using
Real-Time PCR. The fibroblast cells were more efficient in transfection activity
than SCs. Moreover, plasmid pEGFP-N3 had higher capability of transfection
compared to pEGFP-N3-HNP-1 plasmid. The cells confined the poisoning protein in
large particles and non-poisonous protein appeared all over cell in thin
particles. The inflammatory response of SCs to non-poisonous heterogenous
protein was lower than to poisonous heterogenous proteins compared to FCs. The
FasL response of SCs to poisonous protein was faster than to non-poisonous
proteins. It is concluded that Sertoli cells may create strong resistance
against transfection than fibroblast cell, while the former contain large
amounts of harmful/poisonous proteins that may modulate a quick inflammatory
response. The quick inflammatory response may lead to apoptosis in Sertoli cells
which is thought to be a way to get rid of unhealthy cells.