Isolation and Characterization of a Vero
Cell-Adapted Porcine Epidemic Diarrhea Virus in China
H Hu§, TF Liu§, XG Ku, MY
Li, FZ Chen, SY Ye, QG He, XR Meng*
State Key Laboratory of Agricultural
Microbiology, Division of Animal Infectious Diseases, College of
Veterinary Medicine, Huazhong Agricultural University, Wuhan, China;
1 Wuhan Keqian Animal Biological Products Co., Ltd.,
Wuhan, China;
*Corresponding author: xianrongmeng@mail.hzau.edu.cn
Abstract
Since early 2011, diarrhea was rather common
among pig herds in China and the causative agent was proven to be virulent
porcine epidemic diarrhea virus (PEDV). From the small intestine of a piglet
showing mild diarrhea, we isolated an attenuated cell-adapted PEDV strain in a
Vero cell line. The virus was designated as PEDV AH-M. The virus was recognized
in infected cells by a monoclonal antibody against PEDV spike protein. After 10
passages in Vero cells, the virus grew stably to 106.5 TCID50/ml.
Typical coronavirus particles were observed by electron microscopy. The complete
genome sequence of PEDV AH strain was shown to be 27, 953 nucleotides in length.
On the whole genome level, this virus strain shared highly nucleotide sequence
identities with attenuated Korean DR13 (99%) and China SD-M strain (99%). These
data implied that a cell-adapted attenuated PEDV was isolated. We infer that
AH-M could be developed as a vaccine strain while immunogenicity and
pathogenicity of this strain needs further confirmation.