Comparison of Plasma Interleukin-6 and
Interleukin-10 Concentrations between Female Dogs with Mammary Gland
Tumours and Healthy
Marek Szczubiał1*, Magdalena Krawczyk1,
Mariola Bochniarz1, Roman Dąbrowski1, Wojciech
Łopuszyński2 and Renata Urban-Chmiel3
1Department
and Clinic of Animal Reproduction; 2Department of
Pathological Anatomy, Faculty of Veterinary Medicine, University of
Life Sciences, Gleboka 30, 20-612 Lublin, Poland; 3Sub-department
of Veterinary Prevention and Avian Diseases, Institute of Biological
Bases of Animal Diseases, Faculty of Veterinary Medicine, University
of Life Sciences, Akademicka 30, 20-033 Lublin, Poland
*Corresponding author:
marek.szczubial@up.lublin.pl
Abstract
The aim of this study
was to determine the concentrations of IL-6 and IL-10 in plasma of female dogs
with benign and malignant mammary gland tumours and comparison to that in
healthy. The study was carried out on 44 dogs, including 34 dogs with mammary
gland tumours (8 benign tumours and 26 malignant tumours) and 10 healthy
controls. The concentrations of tested cytokines were analysed using a specific
canine ELISA assay. The study showed that both groups of dogs with mammary gland
tumours had significantly higher (P<0.05) concentrations of IL-6 compared to
control group. Similarly as in the case of IL-6, dogs with mammary tumours had
increased concentrations of IL-10 compared to healthy dogs. Only in relation to
dogs with malignant tumours a difference reached statistical significance
(P<0.05). In dogs with mammary carcinomas, the IL-6 concentration increased with
histological grade of tumours and dogs with grade 3 tumours had a significantly
higher (P<0.05) concentration of IL-6 than those with grade 1 tumours. Although
the highest concentration of IL-10 was observed in dogs with grade 3 tumours
significant differences were not found. The strong correlation (r=0.97) was
found between plasma IL-6 and IL-10 concentrations in relation to the health
status of animals. Our results suggest that IL-6 and IL-10 are important factors
involved in a neoplastic process in the mammary gland of dogs. In addition, the
results obtained indicate that circulating IL-6 and IL-10 concentrations could
be helpful for identifying of malignant form of mammary gland tumours in dogs.
To Cite This Article: Szczubiał M, Krawczyk M, Bochniarz M, Dąbrowski
R, Łopuszyński W and Urban-Chmiel R, 2018. Comparison of plasma interleukin-6
and interleukin-10 concentrations between female dogs with mammary gland tumours
and healthy. Pak Vet J, 38(2): 194-198. http://dx.doi.org/10.29261/pakvetj/2018.043