Occurrence and Risk Factors Associated with
Mycobacterium tuberculosis
and Mycobacterium bovis in
Milk Samples from North East of Pakistan
Abdul Basit1, 2, Mubbashir Hussain2,
Muhammad Shahid3,5, Sultan Ayaz4, Kashif Rahim2,
Iqbal Ahmad2, Amin Ur Rehman1, Mohammad
Farooque Hassan3 and Tariq Ali3,5*
1State
Key Laboratory of Agro-Biotechnology, College of Biological
Sciences, China Agricultural University, Beijing, China; 2Department
of Microbiology, Kohat University of Science and Technology (KUST),
Khyber Pakhtunkhwa Kohat, 26000, Pakistan; 3Collage of
Veterinary Medicines, China Agricultural University, Beijing, China;
4College of Veterinary Sciences and Animal Husbandry,
Abdul Wali Khan University Mardan, Pakistan; 5Center of
Microbiology and Biotechnology, Veterinary Research Institute,
Peshawar, Pakistan. *Corresponding author: tariq_ali@cau.edu.cn; dr_khattak@yahoo.com
Abstract
Tuberculosis is a chronic, debilitating and
highly contagious zoonotic disease threatening animals and public health.
This study was designed to investigate the
occurrence ofMycobacterium
tuberculosis and Mycobacterium bovisand to identify the risk factors associated with
the disease in milk samples of dairy animals. A total of 200 milk samples
were collected from lactating
animalsand
milk shops of five randomly selected union councils of district Kohat, Pakistan.
The epidemiological and herd management data was collected on a pre-designed
questionnaire. All milk samples were first
screened by direct microscopy, followed by molecular detection of
M. tuberculosis and
M. bovis using PCR assay. Overall, PCR based prevalence of
tuberculosis causing bacteria was 7.5%.
The high prevalence rate of M. boviswas found in cattle
(6.4%), followed by buffaloes (6.2%), while the
prevalence of M. tuberculosis
was higher in goats (6.3%). A higherprevalence of
M. bovis (15%) was found in
the Khushal Ghar area. Moreover, the prevalence was also high in milk samples
collected from milk shops (7.4%). Additionally, animal herd size and hygienic
measures significantly (P<0.05) contributed to
the prevalence rate.
The prevalence rate in the
age group of >8 years
was found higher compared to other age
groups. Furthermore, thehighprevalence rate was
found in animals having close contact with
other animals in communal grazing and watering. This study concluded that some
factors influence significantly in the prevalence of
tuberculosis. Thus, actual
managemental practices might be helpful in control and
prevention of these pathogenic bacteria.
To Cite This Article: Basit A, Hussain M, Shahid M, Ayaz S, Rahim K,
Ahmad I, Rehman AU, Hassan MF and Ali T, 2018. Occurrence and risk factors
associated with mycobacterium tuberculosis
and mycobacterium bovis in milk
samples from North East of Pakistan. Pak Vet J, 38(2): 199-203. http://dx.doi.org/10.29261/pakvetj/2018.038