The present study focuses on the use of n-hexane, chloroform, ethyl acetate and aqueous extracts of Pteris cretica to confirm its in-vivo anti-inflammatory properties and in-vitro cytotoxic profile. The anti-inflammatory study was conducted by carrageenan-induced edema test at different doses of Pteris cretica extracts (250 and 500mg/kg body weight). At 250 mg/kg dose of chloroform, n-hexane, aqueous and ethyl acetate extracts expressed maximum anti-inflammatory activity at the 2^nd hour with 29.65, 29.06, 27.55 and 26.04% respectively by inhibiting carrageenan-induced edema. Moreover, 500 mg/kg concentration of chloroform extract of Pteris cretica expressed efficient anti-inflammatory activity with 45.3% of inhibition in edema; whereas n-hexane, aqueous and ethyl acetate extracts inhibit the edema at 35.34, 29.06 and 27.55% respectively. MTT assay was used to determine the cytotoxic potential of Pteris cretica extracts on HeLa, and BHK-21 cell lines. Chloroform and aqueous extracts expressed the cytotoxic activity with IC₅₀ of 31.48 and 34.26 µg/mL respectively against HeLa cell lines. However, both chloroform and aqueous extracts exhibit IC₅₀ 108.50 and 55.76 µg/mL against BHK-21 cell line respectively. Chloroform extract is less cytotoxic against BHK-21 cell line as compared to aqueous extract. Ethyl acetate extract was found to be toxic for both cell lines. Based upon the above results, isolation, identification and purification of phytochemicals from Pteris cretica extracts is highly recommended as a possible extension of the current work.

To Cite This Article: Kiran K, Saleem F, Awan S, Ahmad S, Ahmad S, Malik MAA, Akhtar B, Raza M, Peerzada S and Sharif A, 2018. Anti-inflammatory and anticancer activity of pteris cretica whole plant extracts. Pak Vet J, 38(3): 225-230. http://dx.doi.org/10.29261/pakvetj/2018.063